+Marker Data: emg11P16

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 60.5
Annotation
Source Name emg11P16
Source Type genomic
Source Sequence NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNN NNNNNNNNNNNNNNNNNNNNNCCCTAGTTACCTCACCACC AAATTTTGAAATTCAAAAAAAAATCCAGTGTCTCATCAAA AACCCCTCAGTGGTCCATTTTTCCACCAAAACTTCCAGCA TCAATTTCCTTTTCAGGACAGTGAGGCTTTGTGACTGACA AATTGTAGTGCTTACCACCCAAATGGGTATATTGTACTCG AACTCTCCAGAATACGCTGAAATGATCCGTGGAATACAAT TCAGAGGCTTTTGACACAACTTAAGCCCATTTTAGGTAAC CACTTAAAAATAAGTTTTCCTCTATTTTCAATCAACCACA CATTTTCGCCAATTTCTTTTGAGTAGTTTGATTCCGTAGG CAAAAAGCATACTCGACAACCTAATCAGAGGCAGACCACG TGGAGTAGTCGAACGGCATGTGCACCAGTTAACTTAGAAT TCTTCATATGTAAATATATATATAAATATATATATACATA CACACACACACACACACACACACNNNNNCACACACACACA CATATATATACACACACAAAAAAAAAAAAACACACCCCTT AAGGAAGGATGACATATTCAACTGTGCCCCCAAAACACAA AACTAATTGGGGGAACTGATTGAACATGCAACTTAGCCTT GATATCTCGTGTGACATTGTGGGTTTGAATCCAAAACAGC TGCAACCCCTTTTTTTTTGGGCTTCTGCTAGCAAGGCCAN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)5aa(ta)5cat(ac)12nnnnnc(ac)6a(ta)3t(ac)4aaaaaaaaaaaa(ac)3
PCR product (bp) 300
fwd primer emg11P16/U
fwd primer seq. tcaatcaaccacacattttcgcc
rev primer emg11P16/L
rev primer seq. attagttttgtgttttgggggcac
fluor. primer emg11P16/U_PET
dye PET
fluor. Primer seq. PET-acaatcaaccacacattttcgcc
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 290 290
EPL-1 290 290
LS1151 (S.linneanum) 284 284
LS1934 296 296
LS4021 (S. incanum) 286 286
Nakate Shinkuro 290 290
Senryo 2 290 290
Shironasu 290 290
WCGR112-8 290 290
White Egg 294 294
PI 286106 297 297

Population Linkage Group Position
AL2010 E_06 60.5
LW2010 E_06 67.7
LWA2010 LWA_06 64.882
LWAE2012 E_06 70.468
LWF2_2022 E_06 82.0
N28F2 E_06 97.6

DB Program Definition E-Value

EST Name Species Strain