+Marker Data: emf01A03

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 18.7
Annotation
Source Name emf01A03
Source Type genomic
Source Sequence CTCATTATAGCCTATTATAGTTGTGATGATGGTTAGTTAA ATGTGTAGTTAGTGAATTAGTTAGTTAGTTAAATGTTAGT TGGAGAAGGATTAGGAAGATATCCCAGGTGTGTGTACACA TGGTAGTTAGAATGGACCTTCATTAATATATATATATATA TATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TACTGTGCAGAGAGAAGTAATACACTACAATACTTCTTCC TCTTCTTCAATCCTCTTCTCTATGACCTCTCCCTCTTCAA TTCTCTTCATGATATCAGAGCGGGAGTGAGGCATTTCGAT TTCTCAGCTCAATTGATCAAGTATTCGTAGAAATTGACTC AATTTGATAGCAGTAATGGCAGATTCAGCTACCAATA
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)10(tg)17tactgtgc(ag)3
PCR product (bp) 248
fwd primer emf01A03/U
fwd primer seq. tggagaaggattaggaagatatccca
rev primer emf01A03/L
rev primer seq. gctgagaaatcgaaatgcctcact
fluor. primer emf01A03/U_VIC
dye VIC
fluor. Primer seq. VIC-aggagaaggattaggaagatatccca
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 241 241
EPL-1 251 251
LS1151 (S.linneanum) 246 246
LS1934 237 241
LS4021 (S. incanum) 214 259
Nakate Shinkuro 241 241
Senryo 2 241 241
Shironasu 237 237
WCGR112-8 239 239
White Egg 237 237
PI 286106 237 237

Population Linkage Group Position
AL2010 E_06 18.7
EW2009 LG04 92.3
LW2010 E_06 13.6
LWA2010 LWA_06 15.432
LWAE2012 E_06 21.228
LWF2_2022 E_06 16.7
N28F2 E_06 2.7

DB Program Definition E-Value

EST Name Species Strain