+Marker Data: emd02B12

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 37.3
Annotation
Source Name emd02B12
Source Type genomic
Source Sequence ATCGTTCACACTCGGACACCCTGGTGGCAAGGTACTACAC CCCTCACATGGTTGATGTTGTTTACACCCGGACACCCTAG TGGCAAGGTACTACACCCTTCACATGGTTGATGTCGTTCA CACCCGGACACCCTGGTGGCAAGGTACTACACCCCTCACA TGGCTAATGTCGTTCACACTCGGACACCCTGGTGGCAAGG TACTTACCCCTCACATGGTCGTCCGGGTACTTATGACCCT CGGACCGAAAATGGTCTAATATAACACATTATATAAAATA TATATATATGTGTGTGTGTGTGTGTGTATGTGTGTGTGTG TGTGCATGTGTGTGTGTGTGTCCATAAGCTCATTTCATCA TTAAAACAACATACATATCATATCTCATGCTTTGAACCTT CAATTCCTTATGAAACCATAACCAAATACTTTATCAAACA CTTGGCGGATATGTAATAGGGGTAAAAGAGCATGCAATTT AGAGATACAATCATGTAGGAAAATCAGTGGTAAAACCTTT AATAAACACCTTGCAATACCCCGAAGACATTCACATCAAA ACCCCTCATCAATTCCAACATTAGATCCAAGATTTGAAGC TCAAAACATGCTAAAACAACCATATTTTCATGAACATAAA TCACTGTGTAGTAAATTTCTCAATATACAAGACCCATATG TAAATTCAACTTTAAATCATGCAATATTGATGAAAAGAGG GTTTAGGCACCACGGGTGGAAGGACCCAATTGTTCAACAC CACATACCTTAGCTTTTGTGAAGTTCTTGAAGAAGCCTTT AATGG
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)3aa(at)6(gt)9a(tg)8ca(tg)7
PCR product (bp) 234
fwd primer emd02B12/U
fwd primer seq. acttatgaccctcggaccgaaaat
rev primer emd02B12/L
rev primer seq. cccctattacatatccgccaagtg
fluor. primer emd02B12/U_NED
dye NED
fluor. Primer seq. NED-ACTTATGACCCTCGGACCGAAAAT
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 238 238
EPL-1 238 238
LS1151 (S.linneanum)
LS1934 236 236
LS4021 (S. incanum) 238 238
Nakate Shinkuro 238 238
Senryo 2 238 238
Shironasu 238 238
WCGR112-8 238 238
White Egg 238 238

Population Linkage Group Position
AL2010 E_06 37.3
LW2010 E_06 35.8
LWA2010 LWA_06 37.402
LWAE2012 E_06 44.013
LWF2_2022 E_06 41.9

DB Program Definition E-Value

EST Name Species Strain