+Marker Data: ecm074

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 47.6
Annotation
Source Name PED06H13C
Source Type EST
Source Sequence GGGGGCAACTTTCTTCATGCATCTTTTTTTTTTTTTCCCA TTATTCACTTACTTAAATAAGAATAAAGCTTGAACCGCAA ATTGTCCTTTACTTTTTATTTTTTTCTCCATTTTGCTTAC TTTTCTCTCTCTCTAATGGAGCAGTCAGAGAGCTCAACGA CCCACGTTCACCAGGAACCAGAAGAAGAACCAAACGCGAC CGATCAGTTAACGCTTCCACCCTGTTTAACACCTGAAGAA TCCGATGAGCTCAAATCAAGTGTAACCGAGTTCCACGCCT ACCGAGTGAACTCATCCCAATGCTCTTCCCTACTCGCACA ATGCATCCACGCGCCACCTAACGTAGTCTGGTCCATCGTC CGCCGTTTCGAAAGCCTNNNNNCGTCTTTTTGCTGATACA GTTGTGAAATTAAATCTTCAGAAATTAGCCTCCGTTGCTG AAGCTATAGCTCGTGGAGGTAACGGTGAGATTTCATAAAA GTTCAAGTTGATTTTTCTCTTCATCTTAATTTTCTTTCTT TTTCCATATAGCTCCCCTTTTTTGGATTTATGTATTTGGC TGAAATGTTGTGTTTCTAAAAGGATTCACGGAAGAGAACG ACAACCATATCCTTCACCCCTTCAGTTTGATTTTATTTGC GTTATTATTCAATCATAATCCTACTTTATTAGAC
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (tc)5taatggagcagtc(ag)3ctcaacgacccacgttcaccaggaacca(gaa)3
PCR product (bp) 250
fwd primer ecm074/U
fwd primer seq. gggcaactttcttcatgcatcttt
rev primer ecm074/L
rev primer seq. gagctcatcggattcttcaggtgt
fluor. primer ecm074/U_PET
dye PET
fluor. Primer seq. PET-aggcaactttcttcatgcatcttt
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 252 252
EPL-1 253 253
LS1151 (S.linneanum) 253 253
LS1934 253 253
LS4021 (S. incanum) 252 252
Nakate Shinkuro 253 253
Senryo 2 251 251
Shironasu 251 251
WCGR112-8 253 253
White Egg 252 252

Population Linkage Group Position
AL2010 E_06 47.6
ALF2 AL06 53.2
LWA2010 LWA_06 46.785
LWAE2012 E_06 52.747

DB Program Definition E-Value

EST Name Species Strain