+Marker Data: EMB01P20

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 65.4
Annotation
Source Name EMB01P20
Source Type genomic
Source Sequence AACTCTGTTGCTGCCTCGGACAGACTGCTGCCAGCTATCA TGCCATAGCCATAGCTCTTTCTCTCATGGAAGCATATCAC TGAGTGTGCAACATGAGCAGACTACATTGCAGATTCTTCA AGCAAAATTAGGAGCAGATGATCTCCGTACTCAGGTGTGT ACAAATCTAATGCCTGATTTCCGTCTTTATTAATTTACCA CGATGGTTCAACAGTGTATTTCATATCTCATTCTTTTCCC GAATGCTTCAACAGTTAAAATATTATGACGCTGCGGATAA CTCTCTGTCTCTGTCTCTGTCTCTGTCTCTGTCTCTCTGT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCATCGTG GTGCTTTTTTTTTTAAAAAAAAAGAAAATGTATTCAGCAA ATGACATACGCCTTTTATTTTTGTTATACATTGGGCATTT CCCAGTAACAATGAGGGGTCCTCCTTAATAGAAGATAATA AATGACTTGGGTCTTCTTAATAAACCACCTATCTGTTGCT GTATTTTAAAGTTGGAGATTTCACATGCAATATATATGTA GGATGCTGCTGCATGGCTTGAATACTTTGAATCGAAAGCC TTAGAGCAACAAGAAGCTGCAAGAACAGGAGCTCCGAGAC TAGATGCATCCATTGCT
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (TC)16
PCR product (bp) 361
fwd primer
fwd primer seq. cattcttttcccgaatgcttcaac
rev primer
rev primer seq. caaagtattcaagccatgcagcag
fluor. primer
dye FAM
fluor. Primer seq. FAM-CATTCTTTTCCCGAATGCTTCAAC
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
PI 286106

Population Linkage Group Position
AL2010 E_06 65.4
LWA2010 LWA_06 68.67
LWAE2012 E_06 74.285

DB Program Definition E-Value

EST Name Species Strain