+Marker Data: EMB0027

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name EMB0027
Source Type genomic
Source Sequence GGTACATATATGTATATATCTCTCTCTTAAGTAGTATGTA TCTTTCTCTCTCTTACGCTCTCTACTACTTTCTTTCGGCT AACGTTTAACTTCTTGACCGTTACTCTTTTTTGACAAGGG TTCATACTCTCTTGGCCGGAAAAATCCTGGCTTTATCTTC GACGAACCCTTGCCGGATTAGAGATCCAGGGGTTTCCGTC ACCTTCCTCGTCGTCTGTGTCATCATAGTCTCTGTCGTTG TCGTCTGAGTCATCGTCGTAGTAGCCTGCGTCTGTGTTCT TTATCGTTATCATCCTCATCAATGCCGTCTGTGTTCTTTC ATCGTCATCTTGTCTATGCCCTTTGTCGTCGCCTGCGTCT GTGCTCATCGTCATTGTTATCCTCGTCATCGCCATCTGCG TCTGTATTCTTCGTCATCTTCTATGTCTGAGTCTTTTGTC ATCGTCATCATCATCATCATCCTCGTCGGCATCATTTATT CATCATCGTCTACATTATTGTATCCCGCATTGCATCACCG TCGACTGTCCCTTGTTGTCGGTATCAGAGGCGTCTGCTTC TTCACCGGCTTCGTCTTTCTTTCTCCCTTTCTTTACACTT TAGTTTTCCCAGTGGCTTTGCGGGTTCGGCGAGCAGCAGA TTTGTTAATTTTCTGGTCAGCAGCTTGGTAAGATTCTGGC TCGAGTTTTCAGGCAGGCAACAGGTTCGGTGAGGCTATCT CCGATTCCAAGTGATAGCTT
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (TC)5(CAT)5
PCR product (bp) 263
fwd primer
fwd primer seq. tcgtagtagcctgcgtctgtgttc
rev primer
rev primer seq. gtgatgcaatgcgggatacaataa
fluor. primer
dye FAM
fluor. Primer seq. FAM-TCGTAGTAGCCTGCGTCTGTGTTC
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
PI 286106 265 265

Population Linkage Group Position
AL2010 E_06 38.6
LWA2010 LWA_06 37.15
LWAE2012 E_06 43.743
N28F2 E_06 56.9

DB Program Definition E-Value

EST Name Species Strain