| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
GCCACACTTCAGTTCCCAGAGAAT
|
| STS_primer_rev |
GAAGGGAAATGTCTTGCTTTCTGA
|
| amplicon_seq |
NNTCTTATAATACTCACAGAGAGAAACTTAAAAATTCTCAAAATCGTCTACACTCGNAGTATAGTCAAGAGTCAAGACTCTGCAAAAACAGTACATTACAATGGATACTATCTTTATTGGTATTGCAATGCAACGCTAGGTATCAACTCCATAAGTTACCGGTCACAACCCCAACAAGAACATTGATAAAAAGCTAGTTCCTCCCTGTCGCGCTAATGGATCTGGAATGGAAAAGATTGAGTTGGGAAAAAAANNGGGGGGGGGGGGAACCCCCTGCCAATGACCTAATATACCATATTACCCAACAACTCCCATTTGCTAATATCCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACATAACTCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAAAATCAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCCTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCNATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATCACTCCTTTGGAGATCTTGTGCCACTAAAAGATGGAAAATTCTAGATTATCGCCNCAAAATAGAAAGGAACAGAAGAAGACAAGAACCNACATGTATACATGCATAAGTTCATANATTATTTAAAGAAGTAATCTCTTACCTCTAGAGATTCATCATAAACACGCGTTAGTTTACCTGTCNTGAACCAAAATATACGAATCCTGCGATCTGGTGAAGTTACTGAGAACTGTTTACCATCAGGGCTTACCTGGAAATAAAGAAAAGGATAAAAAGTTTTAGAAGACTCTTGTCTACTGATTGGTAAGAATAAGAAATTTGAAACTGCTTACAGCTAACTTGACATGTACATAATTGAAGAATTGTTGCTGTTGTTAAGTGGTACGGTCTGAAGTATGCATAATTTCCAGCAAAAGGTTTTCCTGTAATACCTCTATAGAAGAAACAGCAGTTTTGCATTTCACAATTTCAAAGAGATCAGTATCACTCTTCAATCTAAAATTCACCCTGCAGGCGGAACATAAGACGATAAGCATCAGAGGTGACTATTAGAATTAGGAGCAAGAGACGAACTCTTGAATATAACAAAGAAAGTGAGTGGACATATATACTAAATGATCTATGCTTATCTTCTTAGATAGTATAAGTTAATTATAAGCNGTTGATATAGTACTACTCCATGGAAGTGAATACACACTAACACCACAAAAACGGAGATGGTCTAATAATAAATGCCATATACGGCCTCTTTTGACAAAGCACTATTTTGGAATTTGAAATTGATACNCTCCCCCAACCTCAAAACCCCCCATCTCCATAACAAAAAAAGTAATAGCGAAAAGGGAAACACCAATCTCTTGGATTTCATACACGGATATAATACCAATATTCAGATGGATCATTCTAAAACTCAGGTTGGGGTTTGAGCTCCCTGAAATCAATCATTATTGTCTCCACCAAGGACCAGATATAAGGTAAATGC
|
| SNP_position |
253
|
| allele_1 |
A
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCATGGAAAAGATTGAGTTGGGAAAAAAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCATGGAAAAGATTGAGTTGGGAAAACAG
|
| Common_primer |
ATGGTATATTAGGTCATTGGCAGGGGG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
