| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
AGCAAGGGAAAGCAAAAGGAAAAG
|
| STS_primer_rev |
CATCCTGATCGAACCTCTAGCCAT
|
| amplicon_seq |
CTAATTGCCTTTCCAAGCAAGTGATCCACTAATCTGAACATTGAATCAAATGAACAAATTAGCAAAACTACAATGACATATTCACATAAATGTGCTGACTCAAATATTGCTGCCAATGTCAACCAAATACTGAAAACGTTAACAACAAATGAACAATTTTCTGATCCAAATTTAGCATGATAATATGCATAAGAGCATAATCATAATGCAACAAACCACACATGACAATAAGAACAGATCATTAAATATTAGTTCATCATTCTATATGTCATACGCTTTGATGGTTATCCTATTTTTGCCACATGATGTATAACATGAATAGATCACACAGCTAAACAGACATAGAAAATGACATCCACCATCACACATAAAGCCTTCCTGGACACATTCATAAGCCCACAACGACTACAAACTTGGAAACCACCAACACAAGGAAGATGCTTTATTAAGTGTGCAGGTGAGACTGGTGGAATCCTTCATGATACTGATTGAAACTCTAGAATCCACACAAAGGAATCACGTCCAACACAAATCTCAAGGAAATTACAAAATCAAACATCTAAATTTTCATGTCAATTACTCCCTCTACCCAAATATTTGTCAAGGGGTTTGGAGTAGGAAGGTCAAGACACTTAATTAGACTATGAATTCAAGTATAGATTCATCAAATGCTTAAAACAAAATTCACATATTTGAAGACTAATGAAATGTTCCACAAGTCAAGATAGCTAATGATTCATAATATTTATTTAAAAATTTGAGAAAACCATCATATCA
|
| SNP_position |
43
|
| allele_1 |
G
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAGTTTTGCTAATTTGTTCATTTGATTG
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGTTTTGCTAATTTGTTCATTTGATTC
|
| Common_primer |
TTTCCAAGCAAGTGATCCACTAATCTG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
