| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
ATATCACCGGAGGTTTCAGAAGCA
|
| STS_primer_rev |
ACAGCCACTGGAGTATTTCCAAGC
|
| amplicon_seq |
TCCNTTGCTGTAAAATAAATGCACAAGAACTACTCAATCANAACAAAAAAGAAATAAATGCAAATAAGAGAAGAAAAGTTGAACCTTAAATTAAGAAATAAATGCAAATAGGAGAAGAAAAGTTGAAACCTAAGGAAGTTGCTTACTTCATTACAAAGAGTTTGCTACCTTGAAACAAAAATTCATGAAGAGAGGCTGTAAATGACAATCATGTAGAGAACGAGCTTATTGGAAGCAGCCTAATAATCCACCTTGTTAAGGAAATGGATAGTGGGGCTAACTCAACTTTAAAGGCTCACTCGTGAGGTGAGNTCTATCCAAGATAATATAAGGTTACAATAGTCAGTTCCCTCAACCTATGTGGGATGCTCTAACTCCCTACATGTCCTGGACATTTGGGGCGTGGACAGCATAATATTGTCACAAAACGCTCAAAGAAATCTGGCAAGCTATCATTCTAATTTAAGAATCAGATGCAACCAAGCAAAATGCATCATTCTAGCATCATCACAGATTATAAAGTACNNNNNNNNNTACGCAAATGTTGATTCACTTACTGTTCTCTCCATGTCTATGTACTCCTCCAATACCGCCAGTGACAAATACTGGAATGCCAACCTACAAGATACAGAAGATTTTTTAACAAAAGATCCAGATAAAATACAGAGGAAACAAGAGAGTTATTAGAAAAATCCCTTATCATGCAGTACCTTTGAAGCAAGATACATTGTTGCAGAGACAGTAGTTGCCCCATTCTCCCTGCCTGCCATCTAGGGGAAAAAGTACAAACAATAACTTAAAGAAAACTTAATTTGACAGCTTTCTGGNCAGCTTCCCAAGAATATTTGGNTAGGATCCTAGCAGATATCCAAACATATGTTCCAAACCTTTTGAAAGGAAAACTTGAAGATGAAGCATATATCTAGCTTCTATGGCTTAAANCGTCAGAGACACAAGNTACAACTAAATATGACGATGTCCATAAGATTGCACGTCAANTAATGTTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACNACTTAAGNGCTAACTAACTTAATACAAGTAAGGNTTAAATTAAAGCCGACTACTGAAATGCATATCGCAAAGTCCAACTCTTTNCTCAGTGAATCAGTATGTTTGATGCACAAAAAGCCCCCACATTGATGAGGTAACACATTGATCATTCAAGTAACATGTTATTTCTGCTGTATAAAANGCAGNAAGTCTCCATCCTTTGTTTTCTATTTTATTTGGGAGGGCNAGGGTATGGANGAGGAAGAATGGAGATCAGGGGCAGACCTACTCCATAAACAGCCAGCTCACCAGACATACAGCTTTCAGCTCCGATTTATATATGCATATACATGAAAATCCGTTAAAGTTGGTAAATTTAAGAAAACTGAACAAACTGCTTCAATAAAGTAGTGGATGCAAAGACATGTACTCCTCAAGCTAGTCAGGATTCCAAGCCCATTGCCACCACATCCCACAACTACCCACTCATGGAGGTCAAGTAAATTTTATTTTTAAAATTTAGCTAAACAACATTATTTCCCTTACATTTTGCCTTGAACTTCGTCGCAAAGTACAAAATCATTTTCACGAATATATACATTGTAAAAATAACTAGTGCATAATGTTTATTTTTTGATAACCGAGAAATTCCCATTGTCAGGTGGCACACAGTTTGAAACTCAGCGGATAATAACCCCCCCCCNTACTCTTATCCACAATGGCTAGATGCACACAGTTTGAAACTGAATAAATAATCGATCCGCCCCTCTACTCTTCTCCTGCATGGCTAGTGGCACAGAGTTTAAAANTCTGTGGATCCTCTACCTCTCCAGTTCAATACAAGGCTTTTGCCTTCCACACATCACAAGTTGCAGTCTTACCACTAATATTAAGTAGCACATGATTTTCCTTCAGTTAAATTAATAACAAATAGACAATATAAGCAAACAATAAACATTGAACAGGACGGTCAAAATGCTCTGGTACCATGAGATATTATAGTCGATCCAATGCAACAACN
|
| SNP_position |
158
|
| allele_1 |
G
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCAAGGAAGTTGCTTACTTCATTACAAAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCAAGGAAGTTGCTTACTTCATTACACAG
|
| Common_primer |
GCCTCTCTTCATGAATTTTTGTTTCAAGG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
