| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
CGGCAACGAAACCCTAGTTACATC
|
| STS_primer_rev |
CTTTTGCCTGCAATTTGTCCACTA
|
| amplicon_seq |
NNNNNNACTCCCGAACTAGCCAAGCTCATGCAATGGAAGCTTACTAGAGGCAAATGGAGGTTCTTACTTCTCTGTTCGTCTAGCATATGTCAAAATATATTTATTTTGCTTTCGATAATTTTTAAGGTATATTAATTCGATATAAATATTATGAATTTATAATTATGTGTAAGAAATCTGGTTTCCGAAGCTCTTTTTGTAGTTCGTTTACTATATTTTAAGAATCACCTACATAATATGTGACTCGTTTCAGCTTGTTTGTCTGGTTTTTACTTTTGAATCTCCTGGTCTTAGATTATAGATATGCGGAATGTACCAAAATGCCTTCTAATTTCATGGTCTTAAACATGTCACGCAGAAAGTTAGAATTTAAAGCTTCTAAAAAAGGAAAAAGTCTATCTCTTTGAAATGGACTAAAAAGTAAAGTAATATAAACAAATTGAAATGGAGGGAGTATAGTGAGATGCAGTACTTAAGGCTATGAGAAAAATCCAATTTACATATGATAATTGTCATTCAGGCCTAGGCTGTTGGATTTTGTATCATCACTGGACGATGCTGTTGTGAGATCTGCTTCAGAGAAGGCATTTCAGTCTCTACCTGATATCTCAAAAGCTATTTTGGCACTCACTGTCTTGAAAGGCGTTGGTCCAGCCACTGCCTCGGCTGTCTTGGCTGCTTATGCACCGGATATTGCACCCTTCATGTCGGATGAAGTAAATTTTATCATAACCTTCTTGCTGTGGAAAACTCTATTTGATTATCTGCAATATTATGTTAATTGAAGTGTACTAAGATGTTTGATTTATGTGAAAATGCTGTTTGATAGGCAATGGTTGCTGCAATTGGTAACTCGAAAGACTATACTTTGAAACAGTATCTTGTTTTAGNNNNNNNNN
|
| SNP_position |
341
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCTCTAACTTTCTGCGTGACATGTTTAAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCTCTAACTTTCTGCGTGACATGTTTCAG
|
| Common_primer |
ATGCGGAATGTACCAAAATGCCTTCTA
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
