| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
ATATGGGGCACCTGTTTATGATGC
|
| STS_primer_rev |
AGGATCATTGCCAAGTTATGGCTC
|
| amplicon_seq |
CAAGTTATGGCTCTAGACAAGATTTTTCCAAATGTAAAATATGAATTCTTAGAGACTCCTAAAAGACACATCCAATATAGTGAACTTGAACCTACTACGCAGCTCATCAACAAGCAAGACTAACTTCATTTACATTAACCAGCCTCAAGTCACTTGATGTAGTGAAATAGATCTTTAAGCGGACTGCTTATAGTAAAATNGAAGTGAATGCTTGAGATAATAATATTCCTCCATAAATAACAAAAAACTGGAGCTGAAATCATCTACGTGCTGGATTTCCACCTTGTCCTGATGCATCATATCCAGGACCAACGTTACCGGGCGAGATAGCTGTTCCATGAGGAGCATTTCCTAAAGGTGCTACCTGTCCTTGAGTTGCAACACCGCCTTTGGATGATGCGTCATAGTTAGAGGCCTTGGTAGTATCATAATTGGCTCCCCTTTGCATGTCATAACCAGATCCTCTCTGAGCATCATAAACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
|
| SNP_position |
313
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCCCTGATGCATCATATCCAGGACCAAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCCCTGATGCATCATATCCAGGACCCAC
|
| Common_primer |
ATGCTCCTCATGGAACAGCTATCTCG
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
