| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
TTCTCTGGGAACAATGAAATTGCC
|
| STS_primer_rev |
AGCTCCTTGACAAAGGCTGTTAGC
|
| amplicon_seq |
NNNNNNNNNNNNTTTGAAACTCTACTTTTNCAGGTATNGCTTTTGATTGTGAATTTGTTTTCAAGATAGTTATTTTCTTGTTGAAGGGTGTCTTGGAGCAACAATAAACTTCTTTTTGTGTGACCTATAGGTCGTAGAATTGAGCTGTGGAAGCAACCACTAATGTTTGCTTTAGGATAGTCTGTCGACATCACACCTCTTGGGTTTGGGCCCTTTCCCGGACGTTGCTAACACATGATGCTTTGTGCACCAGACAACCTTTGGTAGTTATTTGCATGTTGAAATGTGTTTACTTTTTTNAGTCTATGTAATTAGGTGTGGTCAGAAGAGTATGTCAGACTAGTTTTTGAATCCTTAATGGCCCTTCCCTTTGAAGCCACAGTCCATAATTACGAAAAGTGAACTTCTGTTGTTGTGTATATTTGTTTGTAATGAAAACCTCAACAATCACCAGGAGGTGTGGGAATGGTAAAGGCTCTTCCACCTTTAACTAGAGGTCTCGGAATCTGAATTATCAGTTGGTTAACCGGAAAACCTTCAAAGGGGCTCAAGAGAATGACTAATGCGGAAACAATTTGCCTCCTTAGGCATAGTGTTTTGATTAGAGACATTAGCAGAGAGCCTTGTAATGAAAGGACTAGTTTACTCTCGACATTTCTGTATACATCACCGCAAGACCAATAACAGAAAGATGGAGAATTGAAAAGAAGTCATAACAAAATGCCATATTCGTGTTTGCAGACTTTTTGCATTTGCAGATGACATTTGCTCTTGTTTGGACTTTGTGTTTATTTCTTGATGCCTCATTTAACATGCAAAATCCTCTGTATAAGTGTTAACATNGAAAGGAAAGATATCATATTTAGCACTGATCCCCCTTATTCTGCTAGTATCTTTGTCAATAAACTGGAATAGCGAGGATTCATATAGCTGACTCTAGCTTGTTTGGGATTGAGTCATAGTTGTTTTCATTTACTGTTTACANCCTTAGTATGTTAATGNNNNNNNNNNNNNNNNNN
|
| SNP_position |
618
|
| allele_1 |
A
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAAACTAGTCCTTTCATTACAAGGCTCT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAAACTAGTCCTTTCATTACAAGGCGCG
|
| Common_primer |
TTTGCCTCCTTAGGCATAGTGTTTTGA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
