| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
CAGTTGTTCATGGAACCATTACAGG
|
| STS_primer_rev |
ACGGATGTTGTTACCCCTCACACT
|
| amplicon_seq |
TAAATGTCGCGGATTCTTCCTTTTAGCGTAATTTTGACAGCCTTCAGATGTGTGTTCATGCTAACATCCACGCCTACGAAAAAATAATACCGTTTCAAAACTTTTAACTCAGAGCTCTCAATTTAGCAAAGTCAACAAGGACAGAATGCATACTTATATGAAAACACATACGGATGTGCTCAAATACATATTTATATGTCGAAAATATATCTAGCAATTTTCTCTAAGTATAAACAACCCCTCATGGAATAAAAGTCAAAAGCATGGAAAGGACAACCAACAGAGAAAGGAAAATGCATATAGGTCTCAAAATATGAAAGTAGGACCAACATTTATGTTTGACTACTCTACTTATCTACAACAGAGATAAATTTCTGCAATACCTAGATAGCCATCAACTAAGCTTTCGTGCAAATTTGACATTTTTGATGCATTCTGCAACCAGCACAAGCCCTGGCACTTGCCATAAGAAGCCTATCAAGGTCTGCTAAGTAGCATCTCTACTGCCAGACAGAAACTAGAATATAATGATTTTTTGGGGGGATAAAGTAATTTTTTAAAAAAAAAATGTCTAGGTACAAAGCATGCCCGTATATTAGAAGTACTAGATTTATCGCACGTGCTTTGCACGTGGTAGCATGATATTGAGTTGCCTACAACTGTAGAGCAGTATGTATACTATATTTATCATAATATATCAGTGGAATTGTCCACATACCCCATTTACCTAATTTATCTCTAGTATCCTTGTACTGCTGAAGAGATTGAAACAGAAGAGATAGATAATGCATACA
|
| SNP_position |
128
|
| allele_1 |
A
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAGTATGCATTCTGTCCTTGTTGACTTT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGTATGCATTCTGTCCTTGTTGACGTG
|
| Common_primer |
ACCGTTTCAAAACTTTTAACTCAGAGC
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
