| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
CTCGCTCTTAAGGCATGGCTCTG
|
| STS_primer_rev |
GGCATTCTCATCTGGGTTCTCAGT
|
| amplicon_seq |
ACGGTAGTGTTGGAGAGCTCCAATTTGTGTAGGTTCCGTAGCTTAGAAGAACCTGCAGTAATCAATCAAGCTAGAGGCATCAGTGGCAAACAGTCGTGATAATGACACTGGATATACGATGCAATACAAGCACTGTTCATTTTTGTATTTCTTAATATCCGGAATATCAAGTAGTAGCGTACCTACAATACACAACTTACTAATGGAAAGGAATCACTATCATAAATTTACTGAATGCCACCCAAAGAGAGAATAAAAAGTATGTAAAGGAAGGAAGCATTTCAGAATCACCTGGTCTCCTTTGCAGTAGTTTCTTCTAGCATAGAAGCAGTATGAGGAAACGTCTTCCTCGTATCCCGCATCTATTAGCCTATGGGCAGAGGCCAATTGAGTTTCTGATTTAAGCGTACTATCTTCTTGTAAATAAAAGGCCTTTCCAGCAGCCTGATCTTCAGAATTAGATGTTTCCTCTCCAGGTGCTGCATAGTTGAAAAAATCTCCCACGGGGCATAAACATCCAGCTTCATCCCAAGGTATATGCATGGTGCGCGAGGATATCTTCAGTAGGAAACTTTTCTCACGTTAAAGCATGAATATAACAAGTAATTGATAGACAATAGTGCTCAAGTACATCGTGAATGAAGACGGACAGAGTGCTAGCTGGATCACGAAAATCAAACACAGCATTGCTATCTTGTATGATTAAGGATTTCTAATCAGAATCACTTAAGTAAAGAATGACATAGTTTTGTTCCATATTAGCATTTGTGAGTCAATCCAACTACGTCG
|
| SNP_position |
284
|
| allele_1 |
A
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCCTACTGCAAAGGAGACCAGGTGATTCT
|
| ASP_primer_L |
GCGGGCAGGGCGGCCTACTGCAAAGGAGACCAGGTGATTCC
|
| Common_primer |
TGAATGCCACCCAAAGAGAGAATAAAA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
