| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
GGACATGGGAGCTGGTAAATCAAG
|
| STS_primer_rev |
CATTAACAGCAGTTTTGGCAGAGG
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGCGGCTTTCTTTTCGTCTACACTGTCGGTGAATAATGCTACGGTGAAATTTGAGATATGGGATACTGCTGGGCAGGAGAGGTACCATAGCTTAGCGCCTATGTACTATAGAGGAGCTGCAGCTGCTATTATTGTGTATGATATCACTAGCTCGGTAAGAAACTATCTAGTTGGGAGNNAATTTGCAATTCANAATGGGTTAATATTGGCTTATTAAAGTATTTTTCTTTTGAGCTGTATATTTGTACTGCGGTCTGCGTGTGAAAAGAGTAGATTTTGTACGAAAAAAACCAACAACTAATGTTGGTCTGCAAATGACTTTTCGGTTTATAAATCAATCCCCTTAAGAGGGGTCTCCTNTTTTTTGCANAAATTTAGAAGGTTGAGGTATTTAATGTCCAATTCAGAAGTGTCAAACAGAGAGTGCACAAAATGCAGTTTACGAATTTCCCCTTCCATAGAAAATTTGTTCCTTAGTGGNNTGGANNGGACTTTGCATAATGAGAGCAGCAGTTGATAAAGTTNNNTTGGTCTCTATTGAATACTACTGAGGAGGACATTTCTACNNNNNNTCATNTTTACTTTGGCTTTTGCTTTACTGATCTTGCTTTNNNNNNNNNNNNNCACAAATCTGTAATATGGATGTCTACATGTTTAAGCANTATGTGNAGATATTTCTNATGCATTTNNNAAATTGANNNNGATGTTGNTCTTTTGTTTTCTGTTAATTTAGNACTCATTNTGCAAGGGCAAAGAAATGGGTGCAGGAATTACAGAAACAANGGTGCTCATCCTTTCAACCTAGATACTTGCTNNNNTTATATAGATAAAAAAATGAAAGTTATAATCTTCTTTCTATTTTGAGTNAANNNNNNACGGAAAGCTCCTTTTTAAGATGTCTAGAAAGTTCTGGAAAAATCATCTTGTTGCCTTANTAATTTTCCTTTATNNNNNNNNGNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCNGAAGGAATGACGGAGCCCTCTATTGNTCGGGAAGGGGAGAATTGTTGAGTGGGTNCCCACTAAATGAAGGGCATGAAAATTATTCATTCAAAGAGGCTGCACATGAATGGTNGAATTCAAAAANNGTTGCGCACATTTTCANTTTAATTTGTCAAAAGTTGTNNAACAACTTTNGACGANNNATTGTTNACTCCAATTCACNNATTTCCTATATAAAGGAGCAGAATTGCTCCTACTTTTGTAAAGTAGAAAAATATAGAGAGAATAAAATACACCGANGTATTTTATCNAGTGAGGTATACCATAAACGATAAAATAAAGTAGTCTGTGAAGAAAAATAGAGTGTGCGCATAAGAAATATTTTCCTAAAAGAAATAGTGAGACTATTTTTTATTTTATCTCTAAGAGCNTATTATTTGTAATGNTAGTTGTACACTTGTGTCTACTATTTGATAAAGAGGAAGAAGAAAATAATAGAAGTGTTGATGTGTGGACGTAGCCCAAANNNNNAGTTTTGGGTGAACCACGTTAAATTTTCTGGTATCTACTTTGCCCTATTTATTCCTCCTAAAATTTTNNNNNNNATTGCTCACTCCGTTGCATGAGCCTCAACACCTTGAAGGTTAAAAATAATCTCTTTAGTTGAAGAGAATGTGAGATTTACATAATGAGAATTCTTAGAGAAAATAACTAGAAGTCGGTGTTCTTATNNNNNNNNNNNNNGAGGTGGATTTNNNNNNNNNNNNNNNNNNNNAGATACCAAAGTCAACAGTAAATTGTTGAAGAGCAAGATAAATAATCGGATCACTAAATTTTAGCTCCCCATATCTACTGNNTTACATTTATGNGAGTGGGGGCAGGANNNTATCTGCCAGTGACAAGGGAATTCGCTAATCTTTGTCTCACTACTTAATCNTTTGTANNNNNNTGTNAGCTAACNNNTTATTTAGGCNNTTCTCTGTATCCAATATGTNGAGGTNNNAGTTCAACCTNNNCTTTATTATTTTNNNNNNNNNNNNNNNGGGACATTAGTTTTCCTAATAGNNNNTTATGGGTCACTTNACAGGAGGCAACGCT
|
| SNP_position |
579
|
| allele_1 |
T
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCAGGAACAAATTTTCTATGGAAGGGGAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGGAACAAATTTTCTATGGAAGGGGAA
|
| Common_primer |
GTCAAACAGAGAGTGCACAAAATGCAG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
