| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
ccgctatcttccatgaccggat
|
| STS_primer_rev |
ggtggtatgctcatgaactcgtca
|
| amplicon_seq |
CCGCTATCTTCCATGACCGGATAAAAGGAGTAAGTTTATAAAGAAATATTTTTTTGCCAAGTTTTCCTTCTTTGATATTTCGCTGTGTTTGTACAAATTGGAGAAGCTCATGTTTTCTGGTTGTCCTTTGAATTTTGCAGTGGAAATGCTGTGATGTTCATGTCAAAGAATTTGACGAGTTCATGAGC
|
| SNP_position |
48
|
| allele_1 |
A
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAAAAGGAGTAAGTTTATAAAGAAATA
|
| ASP_primer_L |
GCGGGCAGGGCGGCAAAAGGAGTAAGTTTATAAAGAAATC
|
| Common_primer |
ACAGCGAAATATCAAAGAAGGA
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
