| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
ggtaggggagcttatggaatcgtc
|
| STS_primer_rev |
ctttagcccacggagaagctgata
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNGCAGATAAAAAAGACGAATTTGTGGTAATGTTTCCTTGTGATTTCAAATGACCAGTCTCATTATTCATGTATAAAATATTTACCGTGTTAAAAAATTACAAAACTTGTGTATATCATCTTTGTTATTTTAGGAATTTGTGAAGGAGAAGGTTAAAGAAGGAAAGAAGAGCAACCGAGAGGTATATTTTGAGAAATTTGTTGCAATCCTATTTCCCTTTTCCGTCACAAAGCTGATGGTTTGCAGTCCTATTATCTTTTTTCATGTTATACTTGATTTGCTTATGTTCATGTCTGTACATATTCTATGGTTTAGGCGAGAGAAGCCAGGAAAAAAGCTATACAAGAGATGAGTGAAGAAGCTAAAGCTGCATTTCAGAGTATGAAGTTCTATAAATTTTATCCGGTGCCCTCACCTGATGCTCCTGATGTATCTCAGGTTAAGGTGAGTATTCTGTTCTAGTCTTTAGTATCTTATAAATAGGTCTAGGCATGATCTCATGTCTGCTAGCTGACGCCTTGTCAGGTCAAAAGCTTCAGTATTGAACTGGCAACANGACTCTGCGTACTCTTTTAAATANTAAACTGTTGATGTTAAAGAATTAAGTTTATGTTTGAGAGAGACAATTTATAATGAGATCTCATGTTTAGTTGATATGTTAAGACTTAGCTATGTTTTGGAAATAAAGTTCTTACCTCTTCCTTTTTCTTTGCAGTCTCCANNNNNNNNNNNNNNNNNNN
|
| SNP_position |
530
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCGGCATGATCTCATGTCTGCTAGCTGAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCGGCATGATCTCATGTCTGCTAGCTGAC
|
| Common_primer |
TGTTGCCAGTTCAATACTGAAGCTTTT
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
