| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
tcctaacagcaaggaggaaggatg
|
| STS_primer_rev |
atcttcaaaggtcttggacgctca
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGANNNNNNNNNNCAAGGATATGGTTAAGTTCAAGGTTCGCTGCTCCAAGTACCTGTACACACTCTGTGTGTCTGACTTTGAGAAGGCGGACAAGCTGAAGCAGTCACTCCCCCCAGGTATGCTTATCTACACTTTCAATACGTTGTTCCAGTTGGCTAGTTTATTTGCNGTATTGCTGCTAATCATCGATGATCACGAATCTCATTGTAGGTTTGAGCGTCCAAGACCTTTGAAGANNNN
|
| SNP_position |
197
|
| allele_1 |
T
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCCCCCCCAGGTATGCTTATCTACACTTT
|
| ASP_primer_L |
GCGGGCAGGGCGGCCCCCCCAGGTATGCTTATCTACACTTC
|
| Common_primer |
GCAAATAAACTAGCCAACTGGAACAACG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
