| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
atgggcggttcgtaacaaaaacta
|
| STS_primer_rev |
caacccatcaacatcttccaaaca
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCTAGTTTGCTGGAGGAAAAAACACTCACGACGTTGATAGAACGACATACTTTAAGCTTTTTGATAGCCAATAAATGGCTAGGGGTACACTGTTTGAAACTCGGTAGATAATAGCCCGCCCCTCTACCCTTGTCCACCGAAAGACCAAGCCTTTTTCTACAACAAGGTCTGAACCCTGTAACGCCACAACCGTGTTGGATCCTCCAAAATTTCAAGAGTCCAAGCAACATAGCTCGGATGAAGGAAATTGTTTGAAATAAATTTGTTATGCTTACTGTAACCGAGCGTCTAAATTTGTTACTTCATTTGGTCACTAAGGTAGGAAACTTTAGTTGGAATGAAAACTAGAAAGAGGAAAAGATGCTGTTTGTGTCAATGCTATTCGGATCATGTATCGTAGGTTTTAAATGCATTAGTCTTAACTTGTTGCTTATCGCGATTTTCAGGCTGAAGTAGGGAGATATTTGATTGGTGATCNAGAATGGGAAATTTATCGTGTCTTACTGGANNNNNNNN
|
| SNP_position |
583
|
| allele_1 |
A
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCGTTATGCTTACTGTAACCGAGCGTCTA
|
| ASP_primer_L |
GCGGGCAGGGCGGCGTTATGCTTACTGTAACCGAGCGTATC
|
| Common_primer |
TCCAACTAAAGTTTCCTACCTTAGTGACCA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
