| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
AACCGCAGCTCACAGACATATCAC
|
| STS_primer_rev |
CGAGATGTGAGCTACAGGATAGTTGC
|
| amplicon_seq |
GTTATCTACAGGATAGTTGCCGGTGGGTATTCTTGGAGAGATTTGTTTCATCCCAACAAACCTAGTAGAGCGGCATTTACTGACTTTAGCTCCTGCATTAGAGGGTTGTGCATCACAGATAGTGAACTTTGTCCCGAGGAAGTTAGATCTGTTACAAAGTTCAGCAAGGGGAAAGTAGTCAGCAAGACGACCATGAAGTAGCAAATGACATACTAGCGCGACAAACCTCAGCTTCCCAATGTAGGTGCTGCTACCCTTGGAAACATCTTCAGCATTTAAAGAAATGATGTAATCTGTGTGTGTTGGCCTTTTACACTTCCGAGCAGCAAGAAGGAACTTACCATCATCATTAACAGCTGTATGAAAACCCAAGTAGTGAAGGTCAGTGCAATATCACTTAGCCTGAATAATGTAAGCAAGAAATCCTACAGAGGTGCGGTATAAGCAAACGAAGCCTCTAATTGTACCAAAAGATACACTGAAAAGGAAAATATAGAAGGAGGGTGCTTAATACATCTAGAAAGAAAGAAGTGAAAATGAAATAAATCCTTATTCACCTTCATTTAGATTAAGGAAGAGGTGATATGTCTG
|
| SNP_position |
516
|
| allele_1 |
T
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCTTTCATTTTCACTTCTTTCTTTCTAGA
|
| ASP_primer_L |
GCGGGCAGGGCGGCTTTCATTTTCACTTCTTTCTTTCTCGG
|
| Common_primer |
GAAAAGGAAAATATAGAAGGAGGGTGC
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
