| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
TTTGCAAGCATGGACAACAT
|
| STS_primer_rev |
CAGCAGGTGTAAAGAGGGATAAA
|
| amplicon_seq |
AATGGGCACCAGCTCTCTATCAGCCACCTCCCGTCGAACTGCATCTTGGAGTGGAAACTTTCCTGATGCTTATTCACCAAATAAATCTGAAATAAAACCACCAGGCTCTAGATTGAGTATGCCTCCGTCTTCATTCATGCCTAATGATGCTAATTCGATGCATTCCTCAATGAATGACGGCAGTTTCAGTGATGACCTTCATGAAGTGGACCTGTGACGCATCAAGATGTATATACCAAGTTTTGCTTGTCATCGTGGTATCCTGCTCGGAATCTCTTCACTTTGGTGGTAACAGAAATGTGGTGTGAGAAGATTGAAGCCCTTTCCTGTCTTCTCACACCACAATATACCCTCCTCTTCTCTTCTTTTTTATTTAGCTACTTTTTTGTAAGATCATTTGTATGTTGAAAGCCAATTTATCTGTAGGGATATAGGTTTTATTTCTTTTACTCTCCCCCGCGGCGGGGGGGAAANNNNNNNNNNNNNNNNNCCCCACCCNNNNNNNNCTCTTTTTTTTGGCCAGGGCCGCAACATATCCAATAGAGGAATTGGCACCAGCTCTCTATCAGCCCCCTCCCGTCGAACTGCATCTTGGAGTGGAAACTTTCCTGATGCTTATTCACCAAATAAATCTGAAATAAAACCACCAGGCTCTAGATTGAGTATGCCTCCGTCTTCATTCATGCCTAGTGATGCTAATTCGATGCATTCCTCAACGAATGACGGCAGTTTCAGTGATGACCTTCATGAAGTAGACCTGTGACGCATCAAGATGTATATACCAAGTTTTGCTCGTCGTCGTGGTATCCTGCTCGGAATCTCTTCACTTTGGTGGTAAGCAGAATGTGGTCAATGAAGATTGGAGCCCTTTCCTGTCTTCTCACACCCCACTACCTCCTCCTATTCTTTTTCCTTTTAATAAGGTACTTTTTTGTAAGATCCAGTTGTATGTGAAAGCCAATTTAGCTGTAGGGATATACGT
|
| SNP_position |
360
|
| allele_1 |
C
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCAGGGCGGCCTCACACCACAATATACCCTCCTCTTC
|
| ASP_primer_L |
GCGGGCCTCACACCACAATATACCCTCCTCTTG
|
| Common_primer |
GGCTTTCAACATACAACTGATCTTACAAAA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
