| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
tgctggtgtaccttatattgttgttgg
|
| STS_primer_rev |
ttagttctcaatctttctgtgcgga
|
| amplicon_seq |
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACATGCCACGGGAAAAATTGAAATTAAAGGTCTGCCGAAAAAGATAATGAGTCAATTCTTTTTGAAACAGATCAAAAAGGAAAGTATGTCATTCTTTTTGAAATGGAGGGANGTATAAAGGCTTTAGAGACANGATCTTTGCTTTTTTCTTTAATCTATGTGCAACTTAGGGATAAGGTTTTCGTAGATTCTACCCTCCCCAAGCACCATTTGTGGTATTACATTAGCTAGGCAGTTTTTGTTGTTGTTATGCTTAGTGACAAGAGTTTCTTCTCATTCATTTCTGCATTAATTTTCCGCAAGCAAGGGAATGTTGCTTTTCTGAAAAATAAATGGAAATGTTGTTGAAAACACTAAAAAATATTTCTTTGTATGTTTGGTGTAAAAACAGAGTACTGTGGCAAATGTCGTCTTTTTCGGCCCCATCTTGGTATATATTCATGAAATTGTTAGTGTGGACTTACAGTGACCGGTTACAACCCAAGTTAAATTTTTTATTGTTTGACAAGTGACTCAAAGTTAAGTGTTATACTGTANNNNNNNNNNNNNNNNNNNNNNNNNN
|
| SNP_position |
442
|
| allele_1 |
A
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCAGTTTTTGTTGTTGTTATGCTTAGTGT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGTTTTTGTTGTTGTTATGCTTAGTGA
|
| Common_primer |
GCTTGCGGAAAATTAATGCAGAAATGA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
