| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
ACAACATAATGGACATGAATGCCG
|
| STS_primer_rev |
TTCAACACTTCAACTCCATCTCGAA
|
| amplicon_seq |
CTACAACATAAATGGACATGAATGCCGGACTTGGGAGGGATTTGGCAGCGGCACTAGACTCTCCTAAACTGTGGGTGATGAATGTTGTGCCAATTAATGCTGAAAACACTCTTGGTGTCGTATATGAGAGGGGTTTGATTGGTATATACCATGACTGGTACGTATCGCTAATGGTTTCTTATCAACTTTTTTTCTTTTTGCGCAAATAGTAAAGCATTTCTGCCTATCCACCTATCCTTGTTTTCAAGAATTCCAAAACCTTCTTCCCTTTATTTACTGAACAACTATAGCATCTAATGAGAAGATTCAAATGAAACATCCAAGACCTCCTATTATTTGCATGTTCGAGGAAAAATCAAGGTTTCATATTGGAGATATTATGTATGCTTTGGCAGTTGTCATTCTTCTGTTACTGTTTTGCACTTGGATAGTTGAGATTATCATTCCTAAAACTTGGACTAATTTCGGTTTTTTTCCTTCTTTCAGGTGTGAAGGCTTCTCTACATACCCGAGAACATATGACCTCCTTCATGCTAATCGCTTGTTCACTCTGTACCAGGACAAGTAAGCATTCTTAAACTCTTTAAAGAAATGGTCTATGCTATTGGATATATTAATACAGAAAAGAAAAAAAGCATTACTGATTCTCAAAGCTATATGATTGTAAAACAGGTGTGATTTTGAAGATATTCTCCTAGAAATGGACCGTGTTCTGCGACCTGAAGGATCAATCATTCTTCGAGATGGAGTTGAAGTGTTGAAA
|
| SNP_position |
94
|
| allele_1 |
T
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCCGACACCAAGAGTGTTTTCAGCATTAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCCGACACCAAGAGTGTTTTCAGCATTAG
|
| Common_primer |
CGGCACTAGACTCTCCTAAACTGTGGG
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
