| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
GTATTGCTAGGAAAGTATTCCCCC
|
| STS_primer_rev |
TGTGTAAGCCAAGTTACCTCCGAA
|
| amplicon_seq |
NNNNNNNNNNNNNNNAGTGTTATACGTGATTTACCTATGTTTAATATTACTGCCTTTTGTTTCAATTTATTTGTCTTACTTTCCTTTCTAATCTATTTCAAAAAGATTGCCTCCTCTTTCCACATGATATGTTTAATACCACGAGATTAAAGGATATTTTGGTACATTCTATGTATCTTTAGTTTAAAACCACAAGATTTTAAAGTCCGTTTTACTTTAACTTCCTGTGAAGTCAAAACCGGGCAAACAAATTGAAATGGAGGGAGTATTATTATTGTGGCTTCTCATTCAAAATGAGGGACAAAATGCTTCTGGGCCAACATTCATGTTTGCTTGTTTTATCTCGAATGCTGAACGAGCATCGATCTATTTGTAAGCTTTGAGGATTATATGATGGCCTGGTTATCAGAGTCAAATCAAGCTCTTCATAAGCTTTAAAAAAAGGGTGAAATGGAACGTGGCAAAAATGTAGGGATGTTCTGAATGTATATGCACTTTCTTAAGTCTGATGCATGTGTTGGCAGTAGACCACTTAGCTAAGGCGAAATATTATCATGCAATTTGATAGTAAAACACTTCATTATGTTGCTTGGATCCTCTAGAAGCTATGCATTTTTTTAAGGATCTTACAAGGGTCCGGCAACATTTTTGGGAGGTCTGAGCAACATAACTGTTTAGGTCCCAGAATGTACAATTCCTTTAATATAAGAGATGACCAAAATTTTATCTCCATGTGTCTTGTCTCTCAGATTCCTGTGACATATGCTGGTGGTGTCACTGTGATGGCTGATCTTGAGAAGATCAAAGTCGCGGGGATGGGGCGTGTGGATGTGACAGTGGGCAGCGCCTTNNNNNNNNNNN
|
| SNP_position |
315
|
| allele_1 |
G
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCTCAAAATGAGGGACAAAATGCTTCTGT
|
| ASP_primer_L |
GCGGGCAGGGCGGCTCAAAATGAGGGACAAAATGCTTCGGG
|
| Common_primer |
CGTTCAGCATTCGAGATAAAACAAGCA
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
