| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
tggcagacaaatttattgaggcag
|
| STS_primer_rev |
tcagaaaaccttgtgagcctttcc
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNCCCCTTCAGGTGATTGTTGAGAGAGCAGAAAAGAGTGATATATCTGAGATTGATAAGAAGAAGTAAGTTGCTTAAGAATCAAGTNGCTCCATTTTGATACATTTGTAACACCACATTTATGTTTCCAAGTTCAACTATATATTGGTTTTNGGCATGAAATTTGCTTTGTAGAATGAGTGTGTGTTATCTCCACTGCTATGTTAGATGTTGCCACACCCATGTCGGATCCTCCAAAAATGTACTATTTGTGAATGCTGCAGCTTTTTGCAGGGGTGGATCCACATGGCGCCTGTCGGGTGCTCAAGCACCCATTGACCCNGAAAAAAGTTGTGTGTATCTATATAGAAAATATGNAGAACTTATATAAATATAAAGATAAGCAGCCATTGGTTCTTGTTGCGCTCCTAAGGCTCAGACCAAGTCCCTAGATGAGGGATCGAATCCCACTGGCAGACACCTGCCAGATGAGCACCCACAATTTTTAAATCTAGAATCTGCCACTGGCTTTTTGTGTTTATTTGTGTGCACATCTTTTAGCACAGGGCTATTATTTATTTGCCTTGCGTTTGTGACATAGGTACCTCGTCCCAGCTGATTTGACTGTTGGACAATTTGTTTACGTGGTTCGAAAGAGGATCAATCTCAGTGCTGAAAAGGCCATATTTGTCTTTGTCAAAAACATTCTACCTCCAACAGGTAAGTACAGTAAGATTAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
|
| SNP_position |
150
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCTCCATTTTGATACATTTGTAACACCAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCTCCATTTTGATACATTTGTAACACAAC
|
| Common_primer |
AACCAATATATAGTTGAACTTGGAAACA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
