| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
CTGCAATTGATGAGCGTCACAG
|
| STS_primer_rev |
CCTGATGTTTACACCCATGGGAAA
|
| amplicon_seq |
CTTCTTGGTTGGCCCATCTGGCCTGATAATTTCCAAAAATGCTTGGTTCTTCACCTTTGAAAAAAGTGTGAATCTCAAATTTCCAATTTCTCTCTTTGTTTTTTTTACCATGGGAAAGGGCACCTGCAAAATCAGGAAATTGAGAATCACACTATTTCAAACTGAGACCAAGCACTTTGGCAATATCAAGCTGATAGGCCAACCAGAAATTGGCAAAAGCTTTAATAATGAGAAAAGTCCCCAGATACAAATGAACCTATAAAAGCAAAGACCACTATTGATCGAGCTGTGTTCATACCTGATCACCAGCATTAGGGCCATCAGTGTGGAAGAGGATTGGCCGGCATCGCTTATCTTCATTCATGAGGCTAGAATTCTGGAAGTGAGCAATGAGTGCAGATTTTCCCTGAATGCGAGCATATGCAAGAGATGCCACCTTCTCACTATTGAATTTTTCCCATTTCTTTCCATTGAAGGCCTGCCAAATATTTTCTCACATTTAATGTAAAAGAAGCAACAATGATAGGAAGTTGCCATTAATTTGAACAAAACTTATTTTGTTTAGGGAGCCAGAACTACATACATGATAAAATGGAATAATGAGAGAAGGATCAGTCATGTTGATAAATGCATAACCTACATTGCATTTGTTCTGCAACAAAACAAACCAAAGTTAGACAGAGTAGAAACAAGATCCAGAATACAAGAAAGCTTTCACCATGGGAAAAGCACCAACCTTGAAATCAATTGGTAGATAGATGAAATCATACGTTCCCCTGTGACGCTACCAAAATAACAAAACGGACTAAGGCAATGGAGAATCACACTATTTTCAAACTGAGACCAGCACTTTGGCAATTATCAAGCTGATAGGCCCAACCAGAAAA
|
| SNP_position |
233
|
| allele_1 |
A
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAGGTTCATTTGTATCTGGGGACTTTT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGGTTCATTTGTATCTGGGGACTTGG
|
| Common_primer |
AAGCTGATAGGCCAACCAGAAATTGG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
