| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
ACGTGCAAGAAGCCAGAGAAAATC
|
| STS_primer_rev |
CTTAGCTTGTCTGCGACACTGCC
|
| amplicon_seq |
NATATTCTCAAAACCCTAGATACCAATTTTTCATTTTCACCATTAAAATTAGGAAAATTTTCATACTCATCTTCATCCTCTGATTGCTTTGAAACTTGACAACAAGGGCCATTTACTTCTCATAGAACTAATTAAGATCCTCTTTTGAGTTGGGGTTTTTAAAGCCAAAATACTCCTTCCGTTCCTTTTTTAGCTGCATTTTTCGTGTCAATTTGAGCAACTAGATTACATTAATNNAATATTTTAATATGAAAATCTTGATATTCAAAAACTATATAAAAAGTACTATAAAGTGCAAGTTTTTTCATATTAGTATGTTAAAAAATATATCTGTTAGTCAAAATTCATATTGTTTATAGGAANCTGTGACTACTAAAAAGGAATGGAGGGAGTAGCTGTTAAGCACTTTTTATAGTATTTGGGTAGAACTGNCTGAACTTTGACCTACATTTTAAGAATTTATTTAGTTCATATGAGTCCCCGGACCCTACTTGTGGGATTCCACCGGATATGTTGTTTATTTATTTCGTAGGAGTATGAGAAGTATTGCAACTTATATTATTTCTCATATAGTTTTCAAACATCAATATATAATTAGTCAAATTGACTCTCACGAAACAAAATGGTGACAAGTAAAAGTGAAGGGAGTGAGCATATTTTATTTCTTTATCCAAGAGAAATGAAAAGTTTTTACAACTAACCATTCATTTGTGTTCCCATCCAGGTATTTATCCTCTGTTTTAGTATTAATTGAATGTGGATTTTGGGAAAATCTAGTACTATTTGAATTNNCCCGACTCGAAGAGTATGTTTATCAATAATTGTTTCCTCTAAACTTCTAGGANGTTTCTGCATTNAGGTCATCATCCATTTTGCTTTAGNATTCTTCTTCTCTCATCTGGNTTTCCAGAATGGAGATAGACCAAGGGCTTAGTTTAGATGACTGGAANTTATGTAGTATATTTGGNTTGTCATTTTTGTTTNGGCGGTCAAANTGTAACCAAGGNCCAAATTTATTNNNNNNNNNNNNNNNNNNNNCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGGAGAAGCTAGAGGTGGCTCGGTGGCCNGATAATATGAGGGAAGCGAGACTGCGATGGTTTGGACATGTGCAGAGAAGGAGTACTGATGGTCCTGTTAGGAGGCGCGAGAGGTTGGTTTTAGGAGCTACGCAGAGGGGTAGAGGTAGACCGAAGAAGTACTGGGGGGAGGTGATTAGACATGATATGGCGGAGCTTGATCTCACAGAGGACATGACCCTAGATAGGAAGNAGTGGAGGTTGCGAATTAGAGTAGTAGGCTAATTGGCAGGGTTAGGTGGATGCATGGGTTAGGTGGAGGACGTGTTTTCTCTTTTTGTGTTCTCATTATCTGTTTTATATGTGTTAGTTGTATCATATTTACATATTGCCATGTGCTATTCATTACTAGTCACTCTTTGTGTTGCCTTCCCTTTCCTTTTTGTCATATCTAGTCCTTTCTGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTCTATGAGGTAGTGGTAAGGTTTGCGTACAGACCCCACTTGTGGGATTTTACTGGGTATGTTGTTGTTNTTGTAAAAGCTTTTTGATTAATTTTGCATTTCAACGTATATATAGCATGGTGTCACTTTAGGCGGGACGTGTCAATGGGCTCTTCACGGTGCCTTTTCCTGTCTCATAATCTCTTGTAAGAGTATTTGCTCAGAATTGAGAAAAAGTTCATTTCATGCTTTTGTGGAACAGGCCATGTGCATTGTGCATTGGCTTTGTTATGTCTAACTTTCTGTTCTCTTCCTTAAAGGTCACTTATGTTACATTGTGCGACTCTTTGTTATTACATATGCTATCTGACATCCGTGAATAACTGTCATGAAATTTTGACAAATATCTGTTATAGTTATTTGTGTCATCAGTTTTCCTTTCTACTTGAGCTTTTTNATGTATCAAATATTTCAGCTTTGCGCAGTAAAATGAAGCAGAAAGCACTCAAAGAATGTGATCGATATACTGCAAAATATGCTGAGTGTGCCTCNNNNNNNNNNNNNNNNNNN
|
| SNP_position |
138
|
| allele_1 |
T
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCCTTTAAAAACCCCAACTCAAAAGAGGA
|
| ASP_primer_L |
GCGGGCAGGGCGGCCTTTAAAAACCCCAACTCAAAAGATGC
|
| Common_primer |
TTGAAACTTGACAACAAGGGCCATTTA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
