| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
tcatatttactcttggcgcactgct
|
| STS_primer_rev |
tgatccaaaacagtcccagttcct
|
| amplicon_seq |
TAGGTATATCCAATATGATTAGAAAATAATATTTGTATTCCATAGTTTATTTGCCACCAACATAAAATCGTAAGATCAAGGTAACCAAGAAACTAGATGGTCAAAGTGATTCAGGCATAAGCAAGATCAAAATTAAAATAACATGCCAAAGCGCAAACACTTGGATAGCACACAACTTAAAAATCTTACATGATTACCCTGCGTCCTGCATTTTTGAAAAGACATATCAACCGCTTTGCATGTGGAAACACAACGCCCCATTTCCTTGCCTACTGTAAATTGAACACTTGTCTATTTGAGTTAGTAAAGCATCTAAGCTAATGAAATTGCTATACCAACAAGATCTTGGACACATTTGTCAATTTTAGATACAAGATGTCAACCCATACACTGTTGTTTATGCCTGAGAAAGAATAGACAAGGGACACAACTAAGGGTGTGGTCTAGTGGTCAATGAAGCGACTTGAGAACCATGAGATATCAAACACAAATCCAAATGGAGGCAAAACCACTAGGTGAATTATTCTCATGTGTCCAAGCCTTGATGGACAAAGCAAGGTACCTTGTGGAATTAGTCGAGGTGTGCAAGCTGGCTCAAGACATCGCGATTGTTTAAAATCGTGGTGTATTTGCAGGAAACGCTGTCTGCAGTTGCTTTTG
|
| SNP_position |
603
|
| allele_1 |
T
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCTGTGCAAGCTGGCTCAAGACAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCTGTGCAAGCTGGCTCAAGACAC
|
| Common_primer |
GCAGACAGCGTTTCCTGCAAAT
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
