| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
AAGGAAGCTGGGCTTTCAAGACTC
|
| STS_primer_rev |
TCAGAACCAATCTCACCAGACCAC
|
| amplicon_seq |
NNNNNNNNNNNNNNNNNNAATGTGCTGCAGATAAATGTTTTCTTGAGAATAACAGGCAAGAGGGATGACGGGTATCATGATTTGGCATCTCTCTTTCATGTAAGTGTTCAACTTCCTTTTTTGTGCTTGAGATTTTGTGTGGTGAGAAATCAGATTATGGTAATGCTTGAAAGAATCCCATTGGAATTATGCTATGCTATCACCCCCTCCGTTTCAATTTGTTTGTCTAGTTTTGACTTGACATAGAGTTTAACAAAGTAAAAAGGACTTTTGAATCACGTGCCTGAAACTAAAGATAAGTAGAATGTACTGAAATGTCTTTTAATCTTGTGGTCTTAAACATGCAATAGAAAGTAAGAATTAAAGAGTTTCATAAAAGGGAAGAAACATTCTTTTTTAACGAACTTGAAAGGAAAGTAAGACAAACAAACTGAAACAAACGGAGTATAATGCAAGTTCTGAAGAATTGCAAGCTCCTGTTATTATCGTTTTTATTAATGAAGTTCTGGAAGAATGAATGATCAAGTGTGTTAGAAGTTTTGAAATGACGAGCCTTTTTTTCTTTCCTTCAGGTAATTAGTCTAGGAGATAAAATAAAGTTTTCGCTCTCACCATCGAAGTCAAAGGATCGTTTATCTACTAATGTTGCTGGAGTTCCACTCGATGAGAGAAATCTGGTAAAGGAAGAAGCTGATATGGGTATCCTCATACTCATCAATTTTAATGATCTGATTAGTATATTCATGATTTTGCCACTCTATTTGCAGATTATAAAGGCCCTCAATCTTTATAGGAAAAAGACTGGAACTGACAATTACTTTTGGGTAAATCTTTAAACTGTTAGCAAAGTAATGAGTTTGGAATAACTTTTGGGTATATTGTGACCTCTTTAACTTTATTACCTGTTTCTCCGGCCAGATTCATCTTGATAAGAACGTGCCAACTGGAGCTGGTCTTGNN
|
| SNP_position |
335
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCACTGAAATGTCTTTTAATCTTGTGGTT
|
| ASP_primer_L |
GCGGGCAGGGCGGCACTGAAATGTCTTTTAATCTTGTGTTC
|
| Common_primer |
TCTTCCCTTTTATGAAACTCTTTAATTC
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
