| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
GGAGATCGGTTGTGGAAATGGTAG
|
| STS_primer_rev |
TGCAGTCACAGGCATACAGAGAGA
|
| amplicon_seq |
NNNNNNNNNNNNCNNNNNNNNNNNNNNTACGGTGAGGAAGAACTGCAGTTGCCTTGAGTGACTTAATTATTTAAAAANTTTTAGGCTGAGGAAGTTTACTACCAAAATTCTCTTTCTGTAAGGTACATAATTATAAGCCGCTAGTCCTATTATTACCTNCTGTTAGCTCTGGTCCTTTGCTTCGATTATCATGTTATCTGTTGTTGCTACTNCTCTTTCTCCTTTTTTATTAGCATGGTTCCTTCATTACTGCTTGTCCTTTGCATACTTGATTTTGATATGTGTTACTTGAGCCGAGATCCTATCCAAAACCACGTCTCTACCTTCACAGCTTAGGGGTAAGGCTGCCCGCTTACACACCACTCTCCCCAGACCCCACTTGTGGGATTACACTGAGTATGTTGTTGTAGTCATATTTTGATATCTCATAATTAATGATATTNAGTGGAGCTATTCTTAATTTGTCATTTTATTCTTTTGTTAGACTTGGAAGTAACCACAAATTTCATGTCCAAACTCAATATATGAGGCTTCTGTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNTANCTTCTATTNAATCTCATTTATATATAACACTGCTGATAGAGTAGAAATATGAAGTTAAGATGAAATCAAAGCTTTTTTGGTTTGATAGTGATGTAGCTACTTGACNTTATTTAAGAGCAATGCTGAAGAATCTTCATTCTTTCTCTAAATTAGTTTAGGTTTATCTGGTTTCATCTCTGTGTTACAACAAGGCTAACATGTACACTTNCTGATTNAGAATTTCTATTTCTATCACGACCTTATTATTCTTATCTCATTGCTGAAACTTTGCTTATCTCTTTAGTGGAAACAAGAGCAT
|
| SNP_position |
300
|
| allele_1 |
T
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCAAGGTAGAGACGTGGTTTTGGATAGGA
|
| ASP_primer_L |
GCGGGCAGGGCGGCAAGGTAGAGACGTGGTTTTGGATATGC
|
| Common_primer |
TGCTTGTCCTTTGCATACTTGATTTTGA
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
