| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
GAGCCTAAAGAGGCATTGAGGGTT
|
| STS_primer_rev |
AATCGCTTCCACAAGCTCCTCTTC
|
| amplicon_seq |
TTTTAGTCTCATAAAAAATCATTGAGGGTTAATGTTCTCTTTGAACACATACAGAAGATGTTGTCCGGTGACACTGCTGTGATTGCTGAGACAGGGGATTCATGGTTCAATTGCCAGAAACTTGAACTGCCCAAGGGATGTGGGTAAGCTCCAAACGACAAGTATTCACGTTTAATGGGTATTTACTTCTGTGATGATGACTGTATTTCATGCTCAGGTACGAGTTCCAAATGCAGTACGGATCTATTGGTTGGTCTGTTGGGGCAACTCTTGGTTATGCACAAGGAGCAAAAGAAAAGAGGGTGCTTGCTTTCATTGGTGATGGTAGTTTTCAGGTAAGAAAGATTGTGTCAACGGAATTCAACACAGAGTTAGCTGTGCTTATCTATTCTATCGCATTCGTATCATCCTGTATTGCAAATACTTGTTGCAGGTGACTGCTCAGGATGTTTCAACAATGCTGCGATGTGGTCAGAGGACTATCATCTTCTTGATAAACAATGGTGGTTATACAATCGAAGTTGAGATTCACGACGGACCTTACAATGTGATTAAGAACTGGAATTACACCGGACTGGTTGATGCAATCCACAATGGGGAAGGAAAATGTTGGACAACAAAGGTGAGGAATTTCTCTATCCCCATACCATTTAACCTGAAGTTAAAAGAATCCTCCGACTAAAGTAATGCATTCTTGTTTTAGGTTCGTTCCGAGGGAGTGGGGGAAAAAAATTTAATAATA
|
| SNP_position |
286
|
| allele_1 |
G
|
| allele_2 |
C
|
| ASP_primer_S |
GCGGGCAGCAAGCACCCTCTTTTCTTTTGCTG
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGCAAGCACCCTCTTTTCTTTTGCTC
|
| Common_primer |
GGTCTGTTGGGGCAACTCTTGGTTAT
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
