| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-misc
|
| STS_primer_fwd |
TAGGAGCAGATGACATCGTCAAGG
|
| STS_primer_rev |
CAAACCTGTGTGTGATCAATGGCT
|
| amplicon_seq |
NNNNNCCTAAACACAATTCGTTGGAAAAATTAGTGCACCGGAAAAAGGGATACAAAAATGAACAAAATGTAAAATGTACGCAGACCTTACCACTACCTTATTGTTAGTGAAGATTAACAAAAGTTTTAGTCGGAACAACAACAACATATTTAGTATAGTCATACAAGTGAGGTCTGGTTTCCAATGAGACCGTCGACTAGGTCAGGAAAATGATCAATATGATCAACATATTCAGTATAGTCAAGTGAGGTCTATGAAGGGTAGAGTTTTTTTATGAGACCCCCGACTTGGTCGTGAAAATGATCAGTATGAGTACTAACAATTAGAGATGAAGCAATTACTAGTTCTTTCTTTTAATATATTTTTTTTTNGTTATTCAGTATAATTTTGATGTACAACAAACTAAAATAGCGACATGGTCAATGAGGATTCAAATAGCACTCTCAACTTAAGAAGGATTCATATCTATCTTTCCACTTCTTAAGGACTCAAGATATATGATGTATTCTAAGTAAAGATATAAGTCGATGGCATTGACCTCTTTGAACACAATTCACCCGAAAATAAGGATAGTAACAAAAGGATATGGTAATGTGCAAGTGTAAGCTTATTGTTACGGAGGATTCGATACACATCTGGAAACATTTTTAAAGAGTCTGAGCAACATAAGCATAAGCAATACCTATTAGTTGGGAATATATTTTAATCATGTTAGAGCGCGTTCACTTTAGATCCTTCATTTTCTAGTTGTCTGTTAGTCCTGCTTAATATTTATATGCCAGTAGAAAATATAAAGAACTTCTAGCACTTCTCATTTTTCATTTTGTATGATATTAGTATGATATATGGTCCGTGAGGATTCATATACCCAACTCCAACTAAGTAAGAATATTGATGCATAGTTGTTGTACCTTGCAGCAGCTGGAGTGAGTGGAACGGTCATCTCGTGATGTCCCATTCCCACCAAGCAAACTTTGCCACCTGGACGAGTTGCACCAAGAGCAGTCGACATGGTTTTGTTAAACCCAGCACAGTCAAAACTCACGTCGATTCCACCTCCCATCGCTTTCTGAATGTTTTCTATATCCGTAGCTACATCCTGTTCAGAAGTACCAGTTAGGGAAGTGGAAAGAAAATCGACTATATCTTCGACTTTGATGTTTGTATATTCACGGGTGAATTTCATGTAATGGTCACTGAGCTTTANNNNCTCAATATAACAGTAAAGTCACAACACTATCTTGTCACAGAACTTTACTGCTATAACTCGTGTA
|
| SNP_position |
582
|
| allele_1 |
G
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCAGCTTACACTTGCACATTACCATATCT
|
| ASP_primer_L |
GCGGGCAGGGCGGCAGCTTACACTTGCACATTACCATATCC
|
| Common_primer |
GGCATTGACCTCTTTGAACACAATTCA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green I
|
