| motif |
(ag)7
|
| PCR product (bp) |
187
|
| fwd primer |
AFES1273-F
|
| fwd primer seq. |
atacaagagcatgcagaaacaa
|
| rev primer |
AFES1273-R
|
| rev primer seq. |
gttgcattgatccacaatcttttga
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
|
| annotation |
hypothetical protein SORBIDRAFT_06g026607 [Sorghum bicolor] >gi|241939588|gb|EES12733.1| hypothetical protein SORBIDRAFT_06g026607 [Sorghum bicolor]
|
| similar marker |
CF448636
|
| product size in J |
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
