+Marker Data: AFRA01B11

Crop bunching_onion
Population SaT03
Linkage Group 7b
Marker Type SSR
Map Position 22.7
Annotation
Source Name AB565018
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2011) Theor Appl Genet 122:501-510
motif (tg)9catgctcttgcgtat(ac)3atacat(ta)3ca(ta)5cacg(tg)5gg(cg)5tatttgtgcgtgcgtgc(gt)3tcataaaaacagttc(at)3
PCR product (bp) 274
fwd primer AFRA01B11-U
fwd primer seq. atcaaagctgcatcaccagagcta
rev primer AFRA01B11-L
rev primer seq. gtttaagtgaatgtggtccgtgtttcct
fluor. primer AFRA01B11-F(PET)
dye PET
fluor. Primer seq. PET-atcaaagctgcatcaccagagcta
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB565018
annotation
similar marker
product size in J
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 265 277
Cho1s-1s-2s-2s-8s 265 277
D1s-15s-10s-8 257 269
F1s-7s-17s-3s-1 257 269
J1s-14s-23s-9 257 269
K1s-5s-2s-2s-4s-5
Chiang_mai 221 233
Kaizuka_Wase
Population Linkage Group Position
SaT03 7b 22.7
DB Program Definition E-Value
EST Name Species Strain