| motif |
(tg)6
|
| PCR product (bp) |
219
|
| fwd primer |
ACE266(PL)-F
|
| fwd primer seq. |
aggttcaacctctgaagtcttg
|
| rev primer |
ACE266(PL)-R
|
| rev primer seq. |
gtttaagttcacatgagcatcccac
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
BQ580097
|
| annotation |
similar to UniRef100_Q2GEM2 Cluster: Dihydroorotate dehydrogenase; n=1; Neorickettsia sennetsu str. Miyayama|Rep:, partial (4%)
|
| similar marker |
|
| product size in J |
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
