| motif |
(ac)10(at)3
|
| PCR product (bp) |
180
|
| fwd primer |
AFA18A02-F
|
| fwd primer seq. |
aaggctacgcaaagaaatcaccaa
|
| rev primer |
AFA18A02-R
|
| rev primer seq. |
gtttacctgcacctgtttgcttgagtta
|
| fluor. primer |
AFA18A02-F(NED)
|
| dye |
NED
|
| fluor. Primer seq. |
NED-acctgcacctgtttgcttgagtta
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB564850
|
| annotation |
|
| similar marker |
|
| product size in J |
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
