| motif |
(tc)18
|
| PCR product (bp) |
167
|
| fwd primer |
AFB02G04-U
|
| fwd primer seq. |
acacgttacagtgggcagaaacaa
|
| rev primer |
AFB02G04-L
|
| rev primer seq. |
gtttgcagagggtggattagtcatttgg
|
| fluor. primer |
AFB02G04-F(NED)
|
| dye |
NED
|
| fluor. Primer seq. |
NED-acacgttacagtgggcagaaacaa
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499472
|
| annotation |
|
| similar marker |
|
| product size in J |
170
|
| product size in Kaizuka-Wase |
160+170
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
