typing_method |
Tm-Shift
|
Marker_Class |
misc_cDNA
|
STS_primer_fwd |
CTGTGGGATGTCAAGTTGTTGGAG
|
STS_primer_rev |
AAATTTGCAGGTCCTTGAAACAGC
|
amplicon_seq |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAAAACAAAANCTGCATTCGATGNAAATATGCAAATACATNCAGAANGCAGACGACGCCTACAGCAATAAACATCCAGTTAATTTTTTTCCTAGCAAATCTATGCTCTCTGGTAANGCACCTCTTACACCAATAAGAACAGATCTTGAAGACAAGTACTTTTAATTCTAGCGATGTGATTCTCTCTGTCCACAAGATTCTCTAATGACAATCCANCCCTACCCTAAACAGGCCGCCTTTGATCGGCGCTTTAATATTTTTCATGGCCAATTTTTTCATAAATAATCAACTTGTTTCTTGGAGAATATCATAGCAGTTTCTTTTAATTTGAAACTGAGAATATCCCAATCTACCTCACCTATCTATGAGGTAGTGGTGCGGACTGCGTACACTTTACCCTTCCCATACCCCACTTTCATAGATAGTGGTGCGGACTGCGTATACTTTACCCTTCCCATACCCCACTTTGCGGTAATATACTGACTAGCAGGCTAGCAGGTTTTGACAGAATATTGCTCGCTGTCCAAACTATGCTGTCTCTTTGTCTTGTGTTTTTTNAGTCTGTGTAGCTCTTTCTCACTGCTGATATTGTGATGCTAGATAAAAGTAATTGGGAAGGNATGGAGCCCGAGCNNNNNNNNNNNNNNNNNNNNNNNN
|
SNP_position |
776
|
allele_1 |
T
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCAGCAGTGAGAAAGAGCTACACAGACTA
|
ASP_primer_L |
GCGGGCAGGGCGGCAGCAGTGAGAAAGAGCTACACAGACTC
|
common_primer |
CGCTGTCCAAACTATGCTGTCTCTTTG
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|