typing_method |
Tm-Shift
|
Marker_Class |
misc_cDNA
|
STS_primer_fwd |
GGCAAGACAAGCTGAAAAGAGGAG
|
STS_primer_rev |
AGCCATGATGGATCTCAACTGCTT
|
amplicon_seq |
NNNNNNNNNNNNNGACAGTAAGTTGCTATCTTTGGCTTTAATTATGTTAGTACGATCTATGAAGTATTTTGTTTCCGGGGGATTTTTGATGTGCAGTTTACTTCTCTTCTTTGATTTTTTCCTGACCATATTAATATTTCTGTACGACATATTTGTTGAGTTCTTCTGTCATTAGTTTTATTGTGCAATTGATATTTCTGTTTTGGCCGAGGGTCTATTAGAAACTGTCTCTCTACCTTCACAAGGTAGGGGTAAGGATGTGCACACACCACCCTCTTGAGACTCCACTTGTGGGATTGCACTGGGTATGTTGTTGTTGTTGATATTTCTGTTTTGGTGTTTCTCTGTTTAGTTTCTCTTGTGGTGTCAAGATAGCTAGGTAGGAGCCCCTCCTTGCTTCTCATTTTTGTTATAGTTGATCTTAAAATTTGAATGAATTCTGTAATTAATTAAAGGAAGACTGAAATGGAGCAAGAACTATGTGCAGAATACCTCTACTGTGAGACAACAGAATTGGTCACCGGCTCTCCCGTCCACATACTTGTGTTAGCTAGATGAAATTATGTACTTGTTTAGTCAGTAATCTATTATGACCACGATTTTGATGGACTGTTCATTTAACTTTTGCCTTGACTATTTAAAATTGTTTCATTGGNTCCTCCTAAATGAATTTTTCTATGAATGGGCACAANGAATCTTCAATGATCATGCTGTTTGTTGTCTTTCTAATTTGAAGTGTTAATGGAGTTTANTAGGACCTCTATGGAAGTAGAGAAAACTCATTTGAGNAGATTTGTATTTTACATAGAGTCCCTAATTTTNATGTGCGCATGCCTCATAANTGATTTANGCATNNGTGAATTTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTTNNNNNNTTTTTTTTTTTCNTCGTCACTTTTCGTATTTTAAAGTCTGGAAGAGAATATNGTTTGCCGAGTTCATTGATATTTAGCATATGGAGATGATCTCCGTCATAATTTTGTCTTTTGTTCCCAGAATCTGTATGAGCAAATTCAAGATTTCTGGATAGACAAAGCCCTATATTACGTAAGTTTTTAAGATAAAAAGATGTGAGAAGCTACTCGGATATGCCCTTGCAATTTCACCATCCGGCAAAGTGAATTGACTAAGTTCTAAGAGATAAAAGTCGCTGACTATAATAGGAGAAGAATGATTCAGTACCTAACAAGAGTTGCTTCTCTCCGAACGGAAACTTGAACTGGAACCAAGATTTAGTTGTACTTGACTGATTAGTGGAGATCTAATTGGTAAGTGAGAGTGATGAGCAAACTAAGTGAGAATCGCTAAATAAAGTACTGCTGATTAAGTTCCATAAATCTATCTTTTTATAAAGAAGGTCCGTGAATCTATTGAGTTCATTTCTAATGTTGTTCTCTTGGAAAAGTTTACTGTTCTCAGTTTTGTGCCACGTAGTTCTATATTCATGAAAGATTAAAAGAAGCATTTACCAGAAACGCGTATCAACACATTAAGAAAAAAGATATAGCTATCACAGGCTTCGTCGTCATCTAATTGTATGGAAGTTAAACCATTGTTCTTTTCTAGGTTTTGGAAGCATTGGACACGTTGAAGAAGAAAACCGATGCACAATCAGAAGAAGTTCTTCCAGTCGAGAAGCTGATAGCGGAGGCATATTCAGCAATCGATAAAGCAGTGAAATCCGGGACACTTCACAGGAACACTGGCGCGANNNNNN
|
SNP_position |
1356
|
allele_1 |
T
|
allele_2 |
C
|
ASP_primer_S |
GCGGGCAAAGATAGATTTATGGAACTTAATCAA
|
ASP_primer_L |
GCGGGCAGGGCGGCAAAGATAGATTTATGGAACTTAATCAG
|
common_primer |
TGATGAGCAAACTAAGTGAGAATCGCT
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|