typing_method |
Tm-Shift
|
Marker_Class |
misc_cDNA
|
STS_primer_fwd |
CTTCTGGATGTCCTTTCAAGTCGG
|
STS_primer_rev |
CCAAGAGATGAACAAATCAAGGGG
|
amplicon_seq |
NNNCAAGTTGGCAAAGCCCGCAAAAAGTACCTCGTTTTTCACTTTTATTTGTTTAAATCTCTTTGCATTTCTTGGTTTTTCCTTATTTTACACAACCCCTTTTCTGATTTTTGTGATTTGGAGATAAAGATTCGTTTTTTTTTTCTATTATGGCAAGCCCCTTTTGTGATTTGAAAATAATTTTTTTTTTTGTTGTTGTTGGATTATGCCAAACCCCTCTTGTGATTTGAAGCTAAAGATTCGTTTTTTATTTCGAATTATGGCAGAACCCTTTTGGGATTTTAGCGTAGAGATGTAATGATAATAATGGAAATCTTAACAGGTACAAGGTGACTTATCCAACAATGTATGCTATTGAATCTGAAAATAAGGATGCTAAGTTCTTTAACTGCGTTCAGGTATGCTATCTTCTTTATAGAGAAACAAGTAGTATTTGTGTTAATTTTCTACACGTGCTTTTGATGTTGTTTGGTAAATTAGGAAATGNTATGGATTTATTGGTGTGTTGATTGCAAANAGGTCATCAGAACTCAATAGAAGCTATGCCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
|
SNP_position |
181
|
allele_1 |
T
|
allele_2 |
C
|
ASP_primer_S |
GCGGGCAAGCCCCTTTTGTGATTTGAAAATAAT
|
ASP_primer_L |
GCGGGCAGGGCGGCAAGCCCCTTTTGTGATTTGAAAATAAC
|
common_primer |
GGTTTGGCATAATCCAACAACAACAAA
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|