typing_method |
Tm-Shift
|
Marker_Class |
misc_cDNA
|
STS_primer_fwd |
ttactattggaattggtggccctg
|
STS_primer_rev |
tgtggtttactatgtcctctacgcca
|
amplicon_seq |
NNNNTTTCTAAATTTGTCTGCATTTTTTGGGATGTGTTCCTAGTAAAATCATTTCAATATTTGCTTCCACTAACAGATTTTCTACCTCATATATCTTAAATTGACAGTAGTAGACTATAAAGCCTAGGTCTTTCGGAAACTGCCTCTCTACCTCCAAGTGGTAGGGGCAAGGTCTGTATACGCTCTACCTTCTCCAGACCCCACTTATGGGATTACACTAGGTATNCTGTTGTTGTTGTTGTAGTGACTCTATAGCGGAGCCTGGATTTTCACTAAGGAGTTCATAAGTGAACATACAAACTAATTGACGGGGGTTCAACATCNACTACATATACATAAAAAAATAATTTNAACTATGTATATATAGCATAATTTTCCATCGAAGCGGGTTCGGATGACCCACCTAGCCCAAGGGTAGCTCCGCCGACTATGACTTGACATTGGTCAGCATCCTAGTTTTTATGAATCTGTCCTATGCAAGATTTTAGCTTCAATATATCAGTAATTTTTACAGTTCTTTAGTATGAAACAAAGTATTTTCCTGGTCTTTCTCTAGTCTTATTTCATTGTTTGTAGCTCTTCATGATTTCCATATGATTGATAGCATTTGATGGTTAACCGAGGAAAGTGGGAAATACACTTCTGCTTTTCATTACGAAAGGGGTGTGTAATGTGTATAACCAGTGAACAAGATGATAAGAGTGAGAAGAGAAAANGTGATCTTANGAAGCGACAACAGGAGGAGGATTTCTTAATAAGTGGGATTTTCTTTCAGTGCAGCAAGCCAAATAAAAACAGCNACCTTTTTTAGATNAAAGAAATTGGAGTACTTCGACCCTGCAGATAGGACNACAGTGTGCTAGAATATGTGGGGCTTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTAGCGAGCACTAAGAAAGTGCAGCTCATGACTCCTCTATTTGCAGAGTAAACAATGATGGGAAGATATAAAATGTCATTGTTTTTCTTGTGAAATTCTGGAATTGTACAAGAATTTTTTAGAAAATTAATCCCTTTGTCCAATTTATGGGTTGACCTTTTTTCTTAAATAGACTATTTTCCATCTCGAAAAGTGAAAATCAACTCGTAGTAAATACCATTTGCATTGTTTGAATTTGATTTGAATTCTTTATACTCTTCTCATATTCAATAAACTTAATTGCAAGAATACATTTTGATATTGATCCTCAATTCCCATGCTNGTCAAAATAGTTTGGTCACTTTGCGACTGATGATTTGAGAGGATATAATAGTATCGGTGTCAGGCATATATCTTTTGTTCTCTTTTAGTTTCTCTTGACTTTGGGGCATTCCCCTCAGCTTCTATCTGAAATTGTTTGACGAAGGTGGAGAAGCTTCTCAATCTTCGTTTATAAAGATACNATCAAATTTGNATGCTTAGTGGCCAAACAGCACAAATATTCTAGGTCAGATAGGTTTTTTNGATGAGACATTCCTTGATATAGAAACTTCTTGAACTGAAAAGGAAAAGGNAAGAGGGGTACAGCTNAAGATTTGCCTAATATTNAGGGCAAGTACTAGGAAAAAGCCACTGTTTTATATGTGAGAATGATATGTTCTTGTGCAAATGCGCTACAGTGCGATTAATAAAAAGCACCACCTTACCGCTGGAAGTTGGTTTGTTCATTGATACTGTTCATTGCTTACCTACGCCACAGGAAAATGTGCCAGGGTCATTTTCATCTATTGCTTCCTAATGCAATATGAATGTGTTGN
|
SNP_position |
178
|
allele_1 |
A
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCTGGGGTCTGGAGAAGGTAGAGCGTAT
|
ASP_primer_L |
GCGGGCAGGGCGGCTGGGGTCTGGAGAAGGTAGAGCGTAC
|
common_primer |
CTCTACCTCCAAGTGGTAGGGGCAAG
|
enzyme |
Stoffel frag.
|
dye |
EvaGreen
|