Typing_Method |
Tm-Shift
|
Marker_Class |
EST-misc
|
STS_primer_fwd |
gaagcaaggaatcacaggtcctaa
|
STS_primer_rev |
gcaaaccatcaccttcaagcttct
|
amplicon_seq |
NNNNNNNNAAGAAATAATTTGATTTTGACTTTGCAAACGAAATATCTCCTAATGTGGTTACTTTATTCATTGACAATGTGGAATCTCTAGAGTCCTGGTCCTTTACTTAAAATTCTGCTAATAAATTATCGTCTTGACATTTGATGTAGCTATTCCTAGTTTGACTACTAATTGTTTAATGGAGCCAATGCAATTAGACAGAAATCTGTTTTTATCGATTCACTTGTGACTAACTTTTCCTTGATTTCTTGATTCCTATGATTGATCCCATATTCTGAATAGTGCCCCTTTAAGAGGGCTTTGGAGATGAAAGGAGAAAAAACTAATGAATGTATATTGCTTCTGTTATTTTTTGGTTGGTCTGTGAATGGCACAGGTACCAGAAACTCTTTCAGACGAGGTTCTTGGAAAGATGGGCGCACCAGCCAAAGGCGATGCACCAATTATCACACCTAATGACCTTGCTGAAAGTGACGGCTTTGTGTTTGGATTCCCAACTAGATTTGGGATGATGTCTGCTCAGTTCAAGGCATTTCTTGATGCTACTGGTGGTCTTTGGAGAACTCAGCAGCTTGCAGGCAAGCCAGCAGGCCTTTTCTACAGCACTGGCTCTCAAGGAGGTGGCCAAGAGACTACCGCGTTAGTCCCTTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
|
SNP_position |
86
|
allele_1 |
T
|
allele_2 |
C
|
ASP_primer_S |
GCGGGCACTTTATTCATTGACAATGTGGAATCT
|
ASP_primer_L |
GCGGGCAGGGCGGCACTTTATTCATTGACAATGTGGAATCC
|
Common_primer |
AGCAGAATTTTAAGTAAAGGACCAGGA
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|