| typing_method |
Tm-Shift
|
| STS_primer_fwd |
GTTTGCTCAAGAGAATATTGCCCC
|
| STS_primer_rev |
ACTCATGGCTAGAGCCCCCACAT
|
| amplicon_seq |
NNTTATTTCCCTCAGGTATGTGCTGTAGTTGCTCTTGTTGTCTGAAGCGTTCTACAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNNNNNNNNNNNNGAAAGANNNNNNNNNNNNNNNNNNNATCANNNNNNNNNNNNNNNNNNNAAATCACTTGATCTCTTCAATTGTTGGAGTCTCATGAAAGCTCCTTTGTTATGTNGTATTCCTTAANGGATATCACGATTCACCATAATATATCTCATAAGTACTTCAAATTTTGGTGGATCCATTTAGGCTAGATTAACTGACAGTGGTGCAACTGGTGCTTTATCAGCAAAACAAAAAAGAGACTGGTGGAACGAGAGTTATTTTTGCCCAGGGTAGGCTATANGTTTATGCACTCAACAACTTCACATAGCAATGGCACTAGCTTAATTAATGAAGAACTGGGTACGTGGGCATACTAGTTGAAGCTTCAAATACTTAATCTGGAATTTTAACAGTATCCGCAACTTGGTTGAATTTTCTCTTATCTATACTGGATTATGTTATTAGGATCAGCTCAAATGATCAATGGCAGAAATCATGTCACACCAAACTGATAACCAACAAATTACAGTTATGAATGCTTAAGACTTTTATATCCGGCATCTTATATGGATCGATAACTTAGAACGCTGGGAAATATACAGGCTTCGATTTAATAATGACAGTTATTCATTTGATTGGAAATAAATTTATTGGAGGAATCTTCCCTTTAAGAAGTTGTGCTGTGAACAAGTTAAGCTTTCTTTTGCTCAAACATAGCCCAACCTTGTTGCGGCTTTCAATTGATCCGATTTTCGCTTGTCAAGGGTTTATCTTTAAATGTGGGGGTGCAAGAGAAGTGGAGCACTATCCTAGTATCTCTGTTCTGTTAATGTTAATCCTTTTTTCTTATTTAATGAAATGTCTCGTGAATAGTGGCTCACTTNGTTATGANNNNN
|
| SNP_position |
386
|
| allele_1 |
A
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCGTTATTTTTGCCCAGGGTAGGCTATAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCGTTATTTTTGCCCAGGGTAGGCTATAG
|
| common_primer |
GCCATTGCTATGTGAAGTTGTTGAGTG
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
