+Marker Data: emj04E17

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 45.2
Annotation
Source Name emj04E17
Source Type genomic
Source Sequence ATTTCACGCTGCTGAAATAGTTTCTTAGAATAGTACCTAG CACAACTCAAGTATTATTGATATGCATATATATATACACA CACACACACACACACACACTCTACTTAAAATAGTATTGTA GTAATATTTATCTTCATTGATTATTTTGACTTATTAATTT TCTCCTGTATTTTATCTTAGTGGGCTAATTCATCACACTG CTCTCAGCATAACTCGTAGTTTTAAGCAACATATGAACAT AACATTCCACTATACTGACAGATGGTACAGCTGAACCAAA GTTTGATATTGATTTGCAATTAAGTCGATGAGTGATACTA TGAATACTTAAAATACAACCCCAACATGTCAGATTAAATT TTATTCAGAAGAATATCTAAACCTAATCAGCAAAGCAATC ATCTAAGAGGAAAC
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)4t(ac)12
PCR product (bp) 213
fwd primer emj04E17/U
fwd primer seq. tcacgctgctgaaatagtttcttag
rev primer emj04E17/L
rev primer seq. cgagttatgctgagagcagtgtga
fluor. primer emj04E17/U_PET
dye PET
fluor. Primer seq. PET-acacgctgctgaaatagtttcttag
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 215 215
EPL-1 219 219
LS1151 (S.linneanum) 212 212
LS1934 214 214
LS4021 (S. incanum) 219 219
Nakate Shinkuro 219 219
Senryo 2 215 215
Shironasu 219 219
WCGR112-8 215 215
White Egg 219 219

Population Linkage Group Position
AL2010 E_06 45.2
ALF2 AL06 50.9
EW2009 LG04 44.0
LW2010 E_06 42.2
LWA2010 LWA_06 43.615
LWAE2012 E_06 51.846
LWF2_2022 E_06 48.1
NAF2 NA06 54.2

DB Program Definition E-Value
TomatoBAC_end BLASTN SL_EcoRI0008E04_SP6_290388 Chromat_file:SL_EcoRI_008_E04-SP6 SGN_GSS_ID:307214 (vector and quality trimmed) 4e-06

EST Name Species Strain