+Marker Data: emf11C06

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 60.7
Annotation
Source Name emf11C06
Source Type genomic
Source Sequence GAACAAACAAGAGTTTGCTCATGTCCAAAACAAAGAATTT TGCTAGCATCAAGCATCTCGTAGAACATATAAATCGGCAA TTATACATGGGCCTCTTCATATTCACAACAAAACGTAGAC ACGAATAACACAAGAGAGAGAGAGAGAGAGAGAGATATGG AAAGCGCCTATAACTGTATGGACAAACATAGCCTGGGATA CGTGAGTTTGGCTCTGCAGCCCCATCATTTTGTTGTTGGA GTTTTCGGGGTAGCATATTTCAGGCTTCTGCTAGCAAGCC TTAAACA
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ag)11
PCR product (bp) 156
fwd primer emf11C06/U
fwd primer seq. tgggcctcttcatattcacaacaa
rev primer emf11C06/L
rev primer seq. aactccaacaacaaaatgatgggg
fluor. primer emf11C06/U_VIC
dye VIC
fluor. Primer seq. VIC-agggcctcttcatattcacaacaa
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 163 163
EPL-1 163 163
LS1151 (S.linneanum) 178 178
LS1934 157 157
LS4021 (S. incanum)
Nakate Shinkuro 163 163
Senryo 2 163 163
Shironasu 163 163
WCGR112-8 163 163
White Egg 155 155

Population Linkage Group Position
AL2010 E_06 60.7
LW2010 E_06 69.0
LWA2010 LWA_06 65.664
LWAE2012 E_06 71.255
LWF2_2022 E_06 83.1

DB Program Definition E-Value
TomatoBAC_end BLASTN SL_EcoRI0112L07_T7_321169 Chromat_file:SL_EcoRI_112_L07-T7 SGN_GSS_ID:337995 (vector and quality trimmed) 1e-20

EST Name Species Strain