+Marker Data: emf11A03

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 42.0
Annotation
Source Name emf11A03
Source Type genomic
Source Sequence NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNN NNNNNNNNNNNNNNNNNNNNCTACTGTTCTCCATTATGTT TAAGGGTCTTTAAACTGATATATATAAATACAATAATAAC ATATTTTGTGAAATTTCACAAGTATGATATGAAAGAATAA AGTGTACACAGATTTTATTACTATTTCATAAAGATAGATA AATTATTTTTCAAAGACCCTAGACTTAAATGCATCCATTC CAAGTAAAAGAAGACCAAAAGATATATATATAACATAGAA ACTACAAGAAAAATAACAATAAGATAGCAAAATAATGTGA TAATCGAAAAGTAATGACTGGCACAACTAGCCCTATGACA AACCTTAAATTGTTACAAGACAAAACAACATTCTACCCAA CTATCCTTCCATTCTAATACATTATTTTCACTTTCTTTTT ATTTACGGTCATGTCTTTGGTATTAAGTTGCACCCTATTC CTATCTAATCATCTCTCCCAAAAATTTTTACACACNNNNN CACACATATATATATATATATATATATATATATATATATA TATTACCCTTATGTATAAGCCAACACGATGAAGTACGAAC ACAACTTTACAAAGTTGTACCAATAGCATAGAAAATTGTA CTACATCATCCATTACTCTTATATGTTGATAAATAAGCAC GTGTATTACATGTGTCCTTGCAGATCCTCCTCCGATTTTC AATTGTTTTGCTTAGATTTTTCAGTCGATTTTTCATCTTT CGTGTTTCTCTTTAGTTGTTACAAAAAATTCACACTTAAT TGAGGGTTCGGAAAAGAAACTCATAAATAGAGGGAGAAAA AATTGATGTAAAATTAGAGAAGAATGATTTGAGGTTCGAG NNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNN
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (tc)3ccaaaaattttt(ac)3nnnnn(ca)3(ta)18
PCR product (bp) 217
fwd primer emf11A03/U
fwd primer seq. caacattctacccaactatccttcca
rev primer emf11A03/L
rev primer seq. gtgttcgtacttcatcgtgttggc
fluor. primer emf11A03/U_PET
dye PET
fluor. Primer seq. PET-atacattctacccaactatccttcca
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 240 240
EPL-1 239 239
LS1151 (S.linneanum) 212 212
LS1934 214 214
LS4021 (S. incanum) 235 235
Nakate Shinkuro 239 239
Senryo 2 247 247
Shironasu 214 214
WCGR112-8 236 236
White Egg 214 214

Population Linkage Group Position
AL2010 E_06 42.0
EW2009 LG04 50.4
LWA2010 LWA_06 40.885
LWAE2012 E_06 47.487

DB Program Definition E-Value

EST Name Species Strain