+Marker Data: emf01I12

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emf01I12
Source Type genomic
Source Sequence CTCTCCATGCAAAAAGGAACTTTTTTATATCCAATTGATG AAGTGTTCAATGGCATATTGCTCTAAGGAGATGAGTCGTT GAAAAGATGCAATTTTGGTAAATGGAGAGAACGTTTCACC ATAATCAAGGTCATAAATCTGTGTATATCTTTCGTTCGAC AAGGTGAGCCTGTGGGAACAAAAGTCATCAATTGCTACAA GTTTGTAGCAATGTCAAAAGTACTTTAAATTAGTTTGCCT ATAAGTTTGATTTGTCAAAATTGTTAGGGAAGGGAAAGTC TAAGAAGTTAGGCATAAGTTTGACACCATCTTTGTCAAAC CAAATCTCACTTACAAACCTACCAAGGTTTGAAATATTGG TTTGTTATGATGTTATTGATGACATAAATAGAGGTAAAAA AAAATTATAAATATGAGAGTTTTGCTCATTTTGTAAAAAC ACCAAAATATAGAGAGCAATAGAAAGAAGAGAGAGTAATC CACAAACTATTCTTAGTTTGTGAGAAAAATAACGTGGGAG TAAAATTGTAGCAAATTTGTATGAAATAAAAGAGTGTTTT TATACAAAGAAGAGTTGTTTTTTTAACACTATCAAGTTGT CATCAATACTATTATATCACATGTACTCCAGTGTAATATT TGGTGACGTTACTCTCTTGTAATTGCTACTTAGCGTGAAT ATTATTCTGGACGTGATTATTATTTTTCCCAACAACGTGG TATCAGAGCCATAGCAAATAATAGTCCACTATCCTTTCAA TACCCCCATCTCACAAGAGAAAATTATGAAAAATGGTGTC TACGAATGAAAGTCATTCTTGGCTCTCAAGATGTTTGGGA CATTGTAGACAAAGCGTATACAAAACCTGTTAATGAAGAA ACTTTGTCCCAAAATGAAAAGGGGATCTAGTTAAAGACAA GAAAGAAAGATCAACAAGCCCTCACTCTCATCCATCAGTG TTTGGATGATGGCATGTTTGAGAAGGTGGCTGATGCAACC ACCTCAAAAGAAG
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ag)3caatagaaaga(ag)4
PCR product (bp) 230
fwd primer emf01I12/U
fwd primer seq. ggcataagtttgacaccatctttgtc
rev primer emf01I12/L
rev primer seq. ctcccacgttatttttctcacaaac
fluor. primer emf01I12/U_VIC
dye VIC
fluor. Primer seq. VIC-agcataagtttgacaccatctttgtc
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 231 231
EPL-1 230 230
LS1151 (S.linneanum) 212 212
LS1934 229 229
LS4021 (S. incanum) 232 232
Nakate Shinkuro 231 231
Senryo 2 231 231
Shironasu 231 231
WCGR112-8 231 231
White Egg 231 231

Population Linkage Group Position
AL2010 E_06 38.6
LW2010 E_06 35.6
LWA2010 LWA_06 36.914
LWAE2012 E_06 43.516
LWF2_2022 E_06 41.9

DB Program Definition E-Value
TomatoBAC_end BLASTN LE_HBa0015I02_SP6_129783 Chromat_file:LE_HBa_015_I02-SP6 SGN_GSS_ID:146614 (vector and quality trimmed) 7e-87
TomatoBAC_end BLASTN LE_HBa0019J21_T7_132174 Chromat_file:LE_HBa_019_J21-T7 SGN_GSS_ID:149005 (vector and quality trimmed) 2e-77
TomatoBAC_end BLASTN LE_HBa0037J15_SP6_80716 Chromat_file:LE_HBa_037_J15-SP6 SGN_GSS_ID:80716 (vector and quality trimmed) 9e-77
TomatoBAC_end BLASTN SL_MboI0019P17_T7_216550 Chromat_file:SL_MboI_19_P17-T7 SGN_GSS_ID:233376 (vector and quality trimmed) 2e-56
TomatoBAC_end BLASTN LE_HBa0043N07_SP6_93948 Chromat_file:LE_HBa_043_N07-SP6 SGN_GSS_ID:93948 (vector and quality trimmed) 3e-52

EST Name Species Strain