+Marker Data: eme36B08

Crop eggplant
Population AL2010
Linkage Group E_05
Marker Type SSR
Map Position 54.0
Annotation
Source Name eme36B08
Source Type genomic
Source Sequence NNNNNNNNNNNNNCCCTGTCCCATCTTCTGATTCATTTTG TGTAGTTAGAGGTCTGTGGACACCTGTGCGTATGGGCTGG GACAGTTTGTATAACTATATATATATGATATGTATATTGG GCGGTTTAACCATAGCGATAACCTTGTCGGCCCGTATGAA TATATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTATGTGTGCTGTTGGCTGGGACTTTTCTAGAT GTAGGGGTCGGATAGTCTATNNNNNGGGGGG
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)7(tg)17ta(tg)3
PCR product (bp) 228
fwd primer eme36B08/U
fwd primer seq. ccctgtcccatcttctgattcatt
rev primer eme36B08/L
rev primer seq. catctagaaaagtcccagccaaca
fluor. primer eme36B08/U_FAM
dye FAM
fluor. Primer seq. FAM-acctgtcccatcttctgattcatt
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 230 230
EPL-1 235 235
LS1151 (S.linneanum)
LS1934 235 235
LS4021 (S. incanum)
Nakate Shinkuro 230 230
Senryo 2 230 235
Shironasu 236 236
WCGR112-8 239 239
White Egg 237 237

Population Linkage Group Position
AL2010 E_05 54.0
EW2009 LG14 12.8
LW2010 E_05 48.5
LWA2010 LWA_05 46.934
LWAE2012 E_05 48.604

DB Program Definition E-Value

EST Name Species Strain