| motif |
(tg)3tt(tg)7
|
| PCR product (bp) |
193
|
| fwd primer |
AFS109-F
|
| fwd primer seq. |
acctatgtctttacctatccaaccaaca
|
| rev primer |
AFS109-R
|
| rev primer seq. |
ccgaatttcaagtgtgtcaagtttt
|
| fluor. primer |
AFS109-F(VIC)
|
| dye |
VIC
|
| fluor. Primer seq. |
VIC-acctatgtctttacctatccaaccaaca
|
| PCR condition |
94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499329
|
| annotation |
|
| similar marker |
|
| product size in J |
190
|
| product size in Kaizuka-Wase |
190
|
| product size in Iki-White |
190
|
| product size in Tender Pole |
190
|
