| motif |
(ac)8a(ta)6
|
| PCR product (bp) |
197
|
| fwd primer |
AFS058_2-F
|
| fwd primer seq. |
atggactgaggtgaaaggatgg
|
| rev primer |
AFS058_2-R
|
| rev primer seq. |
accttttccattgcgtttgg
|
| fluor. primer |
AFS058_2-F(FAM)
|
| dye |
FAM
|
| fluor. Primer seq. |
FAM-atggactgaggtgaaaggatgg
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499322
|
| annotation |
|
| similar marker |
|
| product size in J |
200
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
350+600
|
